NaHCO3胁迫下星星草根中Ca2＋与Ca2＋-ATPase的超微细胞化学定位  

Ultracytochemical Localization of Ca～(2+) and Ca～(2+)-ATPase in the Root of Puccinellia tenuiflora Under NaHCO_3 Stress

作　　者：范玲玲[1] 陈刚[3] 陈义芳[3] 周卫东[3] 戴绍军[1] 孙国荣[2] 

机构地区：[1]东北林业大学生命科学学院,林木遗传育种与生物技术教育部重点实验室,哈尔滨150040 [2]滨州职业学院,滨州256603 [3]扬州大学生物科学与技术学院测试中心,扬州225009

出　　处：《植物学报》2010年第3期337-344,共8页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.30770344、No.30270234和No.30600073);863重大科技专项(No.2007AA021402);教育部新世纪优秀人才支持计划(No.NECT-06-0327)

摘　　要：利用焦锑酸盐和磷酸铅沉淀技术分别对NaHCO3胁迫条件下星星草(Puccinellia tenuiflora)根中Ca2+和Ca2+-ATPase进行超微细胞化学定位研究,旨在进一步探讨Ca2+在NaHCO3胁迫诱导胞内信号转导过程中的作用,以及Ca2+-ATPase活性定位变化与NaHCO3胁迫下星星草抗盐碱能力的关系。结果表明:在正常状态下,根毛区细胞质内Ca2+较少,主要位于质膜附近和液泡中,Ca2+-ATPase主要定位于质膜和液泡膜,有一定活性。在0.448%NaHCO3胁迫下,根毛区细胞质中Ca2+增多,液泡中Ca2+减少,且主要集中于液泡膜附近,质膜和液泡膜Ca2+-ATPase活性明显升高。在1.054%NaHCO3胁迫下,细胞质中分布的Ca2+增多,而液泡中Ca2+极少,Ca2+-ATPase活性也降低。以上结果表明,Ca2+亚细胞定位和Ca2+-ATPase活性变化在星星草响应NaHCO3胁迫的信号传递过程中具有重要作用。We aimed to further investigate the relationships between the localization of calcium and Ca2＋-ATPase and the resistance capacity of Puccinellia tenuiflora against saline-alkali stress. We analyzed ultracytochemical localization of calcium and Ca2＋-ATPase in the root of P. tenuiflora under NaHCO3 stress by potassium pyroantimonate precipitation and a lead-phosphate precipitation technique, respectively. In the normal condition, calcium antimonate precipitation deposits were low in cytoplasm and mainly concentrated within the plasma membrane and vacuoles in the root hair zone. Ca2＋-ATPase had a certain activity and mainly localized in the plasma membrane and tonoplasts. Moreover, under 0.448% NaHCO3 stress, the distribution of Ca2＋ precipitation particles increased in the cytoplasm, decreased in the vacuole, and mainly concentrated near tonoplasts. Meanwhile, the activity of Ca2＋-ATPase was significantly increased in the plasma membrane and tonoplasts. Furthermore, under 1.054% NaHCO3, Ca2＋ precipitation particles continued to increase in the cytoplasm but were absent in the vacuole. The activity of Ca2＋-ATPase was also reduced. All these results highlight that the changes in Ca2＋ localization and Ca2＋-ATPase activity play important roles in P. tenuiflora resistance to saline-alkali stress.

关 键 词：CA2+ Ca2+-ATPase NAHCO3胁迫 星星草 超微细胞化学定位 

分 类 号：Q945[生物学—植物学] S54[农业科学—作物学]