支气管肺发育不良新生儿支气管肺泡灌洗液IL-8 SP-A和TGF-β1的表达  

Expression of IL-8,SP-A and TGF-β1 in bronchoalveolar lavage fluid of neonates with bronchopulmonary dysplasia

作　　者：刘冬云[1,2] 吴静 张小英[1] 封志纯[1] 

机构地区：[1]北京军区总医院附属八一儿童医院,北京100700 [2]南方医科大学研究生学院,广东广州510515 [3]广州市番禺区何贤纪念医院新生儿科,广东广州511400

出　　处：《中国当代儿科杂志》2010年第6期444-446,共3页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金(30973210)

摘　　要：目的了解支气管肺发育不良(BPD)新生儿肺灌洗液IL-8、SP-A和TGF-β1的表达。方法将我院2007年12月至2009年10月诊断为BPD的30名新生儿作为实验组。另外选同期30例非BPD患儿作为对照组,两组胎龄、性别、出生体重无差异。采用非纤维支气管镜支气管肺泡灌洗法进行肺灌洗,用ELISA法测定IL-8、SP-A和TGF-β1的含量。结果 BPD组肺泡灌洗液中TGF-β1、IL-8含量分别为47±15和54±16μg/mL明显高于对照组的34±13和28±13μg/mL(P<0.01)。BPD组SP-A含量明显低于对照组(35±16μg/mLvs42±14μg/mL;P<0.05)。结论 TGF-β1和IL-8在BPD患儿肺灌洗液中有过高表达,可能与BPD患儿肺的异常发育与成熟有关。BPD组SP-A含量较低,提示给予SP-A外源性治疗有可能为BPD的治疗手段之一。Objective To investigate the expression of interleukin 8（IL-8）,surfactant protein-A（SP-A）and transforming growth factor β1（TGF-β1）in bronchoalveolar lavage fluid（BALF）of neonates with bronchopulmonary dysplasia（BPD）.Methods Thirty neonates with BPD and 30 gestational age-,gender-,and birth weight-matched neonates without BPD（control group）were enrolled from December 2007 to October 2009.Non-brochoscopic bronchoalveolar lavage was performed.The levels of IL-8,SP-A and TGF-β1 in BALF were measured using ELISA.Results The levels of TGF-β1（47±15 μg/mL vs 34±13 μg/mL）and IL-8（54±16 μg/mL vs 28±13 μg/mL）in the BPD group were significantly higher than those in the control group（P0.01）.In contrast,the contents of SP-A in the BPD group were significantly lower than those in the control group（35±16 μg/mL vs 42±14 μg/mL;P0.05）.Conclusions The increased expression of TGF-β1 and IL-8 in BALF may be involved in abnormal lung development and maturation in neonates with BPD.The low expression of SP-A in the BPD group suggests that the exogenous SP-A administration may be an option for the treatment of BPD.

关 键 词：支气管肺发育不良 支气管肺泡灌洗液 白介素8 肺表面活性相关蛋白A 转化生长因子β1 新生儿 

分 类 号：R722.1[医药卫生—儿科]