紫草素抑制单核细胞募集及其对人鼠嵌合内异症模型的影响  

Shikonin Inhibits Mononuclear Chemotaxis and Its Effect on Animal Endometriosis Model

作　　者：袁冬平[1] 史颖莉[2] 顾林[2] 陈捷[2] 龙军[1] 许立[1] 

机构地区：[1]南京中医药大学药理教研室,南京210046 [2]南京医科大学附属南京妇幼保健院,南京210004

出　　处：《生殖与避孕》2010年第5期289-293,304,共6页Reproduction and Contraception

基　　金：江苏省自然科学基金(BK2006577);南京市医学重点科技发展项目(ZKX08004);南京医科大学科技发展基金重点项目(07NMU2022)

摘　　要：目的:探讨紫草素影响子宫内膜异位症(EMs)小鼠模型RANTES募集单核细胞、抑制异位内膜生长的作用机制。方法:建立人鼠嵌合型EMs动物模型,设大(10mg/kg)、中(5mg/kg)、小(2.5mg/kg)剂量紫草素治疗组,治疗28d。另设PBS阴性对照组、达菲林阳性对照组,采用形态学方法比较紫草素对EMs小鼠模型移植人子宫蜕膜生长抑制的情况;体内趋化实验评价紫草素对RANTES募集单核细胞的影响。结果:不同剂量紫草素及达菲林均能抑制小鼠体内人子宫蜕膜的生长,与PBS组比较,差异显著(P<0.05)。其中大、中剂量组异位灶缩小最显著(分别为P=0.000和P=0.001),其次为达菲林组(P=0.003)和小剂量组(P=0.011),各组间比较差异无显著意义(P>0.05)。紫草素明显抑制rhRANTES对U937细胞的趋化作用(P<0.05)。结论:人鼠嵌合型EMs动物模型可用于EMs的研究。紫草素能抑制异位灶生长,该作用可能通过抑制趋化因子募集单核细胞至腹腔,减轻腹腔炎症而起作用。Objective：To evaluate the effects of shikonin on mononuclear chemotaxis and endometriosis development in SCID mouse model.Methods：SCID mice were implanted with normal human endometrium tissue fragments.After 2 weeks of lesion establishment,mice were treated with shikonin at different doses（10 mg/kg,5 mg/kg,2.5 mg/kg,） for 28 d,after which lesion size was recorded and morphological method was used to evaluate the development of implanted human endometrium.Furthermore,in vivo chemotaxis assay was conducted to elucidate the effect of shikonin on chemotaxis of U937 cells by RANTES.Results：Compared with PBS group,lesion sizes in shikonin-and diphereline-treated mice were significantly decreased.Although the high and middle doses of shikonin caused greater regression（P=0.000 and P=0.001,respectively）,no significant differences were observed between the treated groups.Shikonin inhibited greatly the chemotaxtic effect of rhRANTES on U937 cells（P0.05）.Conclusion：The SCID mouse is an attractive animal model for the study of endometriosis.Shikonin can effectively inhibit the growth of endometriotic lesions,maybe by its inhibitory effect on chemokine to recruit monocyte.

关 键 词：紫草素 子宫内膜异位症(EMs) 单核巨噬细胞 腹腔炎症 

分 类 号：R285.5[医药卫生—中药学]