生物信息学分析辅助合成、表达噬菌体Chp3 Vp1全基因的研究  

Synthesis and Expression of Chlamydiaphage Chp3 Capsid Vp1 gene by Bioinformatics

作　　者：姚卫锋[1,2] 刘原君[1,2] 冯斌[1,2] 齐蔓莉[1,2] 李燕[1,2] 田敬群[1,2] 刘全忠[1,2] 

机构地区：[1]天津医科大学总医院皮肤科 [2]天津性传播疾病研究所,天津300052

出　　处：《中国皮肤性病学杂志》2010年第6期505-508,共4页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(30671879)

摘　　要：目的取得在衣原体感染中具有重要意义且高度保守的衣原体噬菌体Chp3衣壳蛋白Vp1蛋白。方法以生物信息学技术分析设计、合成Chp3Vp1基因,并对Vp1基因进行克隆、表达、提纯。结果获得了经过测序鉴定的Vp1基因,其蛋白序列与Genebank相一致。诱导表达后,SDS-page和Western Blot均显示获得约55kDa处的衣原体噬菌体Chp3衣壳蛋白Vp1。结论顺利获得高度保守而特异的衣原体噬菌体Chp3衣壳蛋白Vp1。Objective To get the Chlamydiaphage Chp3 Capsid Vpl protein which is highly conserved and important for persist Chlamydial infection by bioinformatics. Methods To construct the whole Chp3Vpl gene by bioinformatics, then link the gene with the plasmid vector in vitro, transforming the recombinant gene into bacteria,ex- press and purify the recombinant Vp1 protein. Results The Gene was proved by Sequence analysis of Vpl, the eapsid protein was correction which was confirm by SDS-page and Western Blot. Conclusion We got the Chp3 Capsid Vpl protein that may be a method to find new Chlamydia-phage successfully.

关 键 词：生物信息学 衣原体噬菌体 VP1蛋白 表达 

分 类 号：Q78[生物学—分子生物学] R691.3[医药卫生—泌尿科学]