小鼠念珠菌性阴道炎模型中TSLP表达及其与IL-2,IL-10关系  

Relationship between Thymic Stromal Lymphopoietin (TSLP) and IL-2 and IL-10 in Mice with Vaginal Candidiasis

作　　者：陈嵘祎[1] 樊翌明[1] 高涛[1] 杨艳平[1] 张国学[1] 

机构地区：[1]广东医学院附属医院皮肤科,广东湛江524001

出　　处：《中国皮肤性病学杂志》2010年第6期509-511,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的探讨胸腺间质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)在小鼠念珠菌性阴道炎发病机制中的作用。方法采用皮下注射雌二醇和阴道内接种白念珠菌悬液建立Balb/c小鼠念珠菌性阴道炎模型,用激光扫描共聚焦显微镜和ELISA检测实验组和对照组小鼠阴道组织中TSLP,IL-2,IL-10表达。结果实验组小鼠阴道组织中TSLP表达明显高于对照组(P<0.01);IL-2水平在接种后7d明显增加(P<0.01),14d时出现明显下降(P<0.01);IL-10含量在接种后7d、14d均明显高于对照组(P<0.01)。TSLP仅与IL-10表达呈显著正相关(P<0.01)。结论 TSLP表达上调可能在念珠菌致病中起重要的免疫调节作用。Objective To investigate the role of thymic stromal lymphopoietin （TSLP） in the pathogenesis of vaginal can- didiasis in mice. Methods Balb/c mice were used to establish the model of vaginal candidiasis by subcutaneous injection of estradiol and vaginal perfusion of Candida albicans inocula. TSLP, IL-2 and IL-10 expression of the vagina in experiment and control groups was determined by laser scanning confocal microscopy and ELISA, respectively. Results Vaginal TSLP expression and IL-10 level were significantly higher in experi- ment group than those in control group （P 〈 0.01 ）. IL-2 concentration increased on the 7th day but decreased on the 14th day （P 〈0.01 ）. Both the contents of IL-10 after 7d and 14d of vaccination were obviously higher than the control group（ P 〈 0.01 ）. Conclusion The overexpression of TSLP may play an important immuno- modulatory role in the eandidal pathogenesis.

关 键 词：阴道炎 念珠菌 小鼠 胸腺间质淋巴生成素 

分 类 号：R519.3[医药卫生—内科学]