绞股蓝皂甙对光损伤小鼠表皮中抗氧化应激酶的影响  

The Effect of Gypenosides to Antioxidative Stress Enzyme in the Epidermis of Photo-damage′s Mice

作　　者：胡蓉[1] 邓丹琪[1] 王医林[1] 袁李梅[1] 刘流[2] 

机构地区：[1]昆明医学院第二附属医院皮肤性病/风湿免疫科,云南昆明650101 [2]昆明医学院第一附属医院头颈颌面外科,云南昆明650031

出　　处：《中国皮肤性病学杂志》2010年第6期517-519,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的观察不同浓度绞股蓝皂甙(GP)霜对小鼠皮肤光损伤模型中氧化应激相关酶活性的影响。方法采用多次UVB辐射Balb/C小鼠建立6组皮肤光损伤的动物模型,用可见光分光光度法和紫外线分光光度法测各组皮肤组织中超氧化物岐化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)的含量。结果 1.5%绞股蓝皂甙霜组Balb/C小鼠表皮中SOD、CAT、GSH-Px、GR活力高于UVB组(P<0.05),与维生素E霜组比较无明显差别。结论 1).1.5%绞股蓝皂甙霜可使UVB照射后光损伤Balb/C小鼠表皮中SOD,CAT,GSH-Px,GR活性得到恢复,其抗氧化作用与维生素E霜相似。2).1.5%绞股蓝皂甙霜有抗氧化损伤的作用,其保护的机制可能与清除氧自由基,提高部分抗氧化酶活性有关。Objective To observe the effect of cream gypenosides with different concentration on the enzymatic activity concerned with oxidative stress on photo-damage. Methods Six groups of photo-damage＇s model mice were set up and the levels of superoxide dismutase （SOD） , catalase （CAT） , glutathione peroxidase （GSH-Px） , glutathione rednctase （GR） in its epidermis were measured with visible speetrophotometry and ultraviolet spectrophotometrie methods. Results The levels of SOD, CAT,GSH-Px and GR in Balb/C mice＇s epidermal tissue of 1.5% gypenosides group were higher than those of the UVB group （P 〈 0.05 ）. The significant different level of SOD, CAT, GSH-Px, GR was not observed between 1.5% gypenosides group and the vitamin E group. Conclusion 1. The levels of SOD, CAT, GSH-Px and GR in mediated photo-damage mice increased after topical 1.5% gypenosides treatment. The anti-oxidase effect of 1.5% gypenosides is similar to that of vitamin E. 2. The topical application of 1.5% of gypenosides has the protective effect on UVB mediated skin damage. The protective mechanism may be related to the eliminating the free radicals and increasing the levels of SOD, GSH-Px, CAT, GR to inhibit the oxidative damages caused by UVB.

关 键 词：光损伤 超氧化物岐化酶 过氧化氢酶 谷胱甘肽过氧化物酶 谷胱甘肽还原酶 绞股蓝皂甙 

分 类 号：R758.14[医药卫生—皮肤病学与性病学] R-332[医药卫生—临床医学]