氯沙坦钾对大鼠腹膜透析模型腹膜纤维化组织中TLR4表达的影响  

Effect of Losartan on the Expression of TLR4 in Peritoneal Fibrosis of Rat Peritoneal Dialysis Model

作　　者：胡春艳[1] 方敬爱[1] 孙艳艳[1] 张晓东[1] 李秀秀[1] 

机构地区：[1]山西医科大学第一医院肾内科,太原030001

出　　处：《中国中西医结合肾病杂志》2010年第5期391-393,共3页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的:探讨氯沙坦钾对大鼠腹膜透析模型腹膜纤维化组织中TLR4、IL-6、IL-8表达的影响。方法:30只雄性SD大鼠随机分为正常对照组、腹膜纤维化模型组(模型组)、氯沙坦钾治疗组(治疗组),每组各10只。正常对照组不予任何干预,模型组给予4.25%腹膜透析液腹腔注射(100ml.kg-1.d-1),治疗组给予4.25%腹膜透析液腹腔注射(100ml.kg-1.d-1),同时给予氯沙坦钾片灌胃(30mg·kg-1.d-1),于4周末杀检大鼠,做腹膜平衡试验,准确量取超滤量,留取0和4h血清与腹腔流出液标本,计算葡萄糖转运量,4h后,取壁层腹膜组织作形态学观察,用免疫荧光的方法检测TLR4、IL-6、IL-8在脏层腹膜组织中的表达。结果:与正常对照组比较,模型组大鼠壁层腹膜厚度明显增厚(P<0.01),超滤量下降(P<0.01),葡萄糖转运量升高(P<0.01)。与模型组相比较,氯沙坦钾治疗后大鼠壁层腹膜变薄(P<0.01),超滤量增加(P<0.01),葡萄糖转运率量下降(P<0.01);正常腹膜组织中TLR4、IL-6、IL-8在间皮细胞中呈弱表达,模型组与正常组相比,TLR4、IL-6、IL-8表达明显增多;治疗组与模型组相比,TLR4、IL-6、IL-8蛋白表达明显下调。结论:氯沙坦钾能够下调腹膜纤维化大鼠腹膜组织TLR4、IL-6、IL-8表达水平,改善腹膜的超滤和转运功能。Objective：Establish a model of peritoneal fibrosis in rat related to peritoneal dialysis to investigate the effects of losartan tablet on expression of TLR4,IL-6,IL-8 on peritoneum in the model.Methods：Thirty male SD rats were randomly divided into the following three groups：control group （n=10）,model group （n=10） and losartan-treated group （n=10）.Rats were treated with daily intraperitoneal injections of 4.25% dianeal （100 ml/kg） over a period of 28 days.rats were fed with Losartan tablet （30 mg·kg^-1·d^-1） besides injections of dianeal in losartan treated group,rats in control group were fed normolly,All rats were sacrificed at the end of 4 weeks.Peritoneal equilibrium test （PET） was performed.The ultrafiltration was detted,and mass transport of glucose was also calculated,The parietal and visceral peritoneum samples were harvested for regular histological evaluation after PET.The expression of TLR4,IL-6,IL-8 in peritoneal tissue was detected by immunofluorescence.Results：Compared with the model and normal group,after treated with losartan,the thickness of parietal perineum was shorted clearly（P0.01）,the ultrafiltration was improved,and mass transport of glucose was also decreased（P〈0.01）.A significant downregulated of TLR4,IL-6,IL-8 was also observed in the treated rats.Conclusion：Losartan can down-regulated the expression of TLR4,IL-6,IL-8 during peritoneum in the treated group,reverse the peritoneal sclerosis partly,and improve function of ultrafiltration and glucose transfer.

关 键 词：氯沙坦钾 腹膜纤维化 TLR4 

分 类 号：R692.5[医药卫生—泌尿科学]