Genetic analysis and gene fine mapping of a rolling leaf mutant (rl_(11(t))) in rice (Oryza sativa L.)  

作　　者：ZHOU Yong FANG YunXia ZHU JinYan LI ShengQiang GU Fei GU MingHong LIANG GuoHua 

机构地区：[1]Jiangsu Key Laboratory for Crop Genetics and Physiology/Jiangsu Key Laboratory of Plant Functional Genomics, Yangzhou University, Yangzhou 225009, China

出　　处：《Chinese Science Bulletin》2010年第17期1763-1769,共7页

基　　金：supported by the National Basic Research Program of China (2005CB120807 and 2006CB101703);National Natural Science Foundation of China (30671282);Key Program of the Bureau of Edu-cation, Jiangsu Province, China (05KAJ2012)

摘　　要：The exploration of new genes controlling rice leaf shape is an important foundation for rice functional genomics and plant architecture improvement. In the present study, we identified a rolling leaf mutant from indica variety Yuefeng B, named rl11(t), which exhibited reduced plant height, rolling and narrow leaves. Leaves in rl11(t) mutant showed abnormal number and morphology of veins compared with those in wild type plants. In addition, rl11(t) mutant was less sensitive to the inhibitory effect of auxin than the wild type. Genetic analysis suggested that the mutant was controlled by a single recessive gene. Gene Rl11(t) was initially mapped between SSR markers RM6089 and RM124 on chromosome 4. Thirty-two new STS markers around the Rl11(t) region were developed for fine mapping. A physical map encompassing the Rl11(t) locus was constructed and the target gene was finally delimited to a 31.6 kb window between STS4-25 and STS4-26 on BAC AL606645. This provides useful information for cloning of Rl11(t) gene.The exploration of new genes controlling rice leaf shape is an important foundation for rice functional genomics and plant architecture improvement. In the present study, we identified a rolling leaf mutant from indica variety Yuefeng B, named rllm）, which exhibited reduced plant height, rolling and narrow leaves. Leaves in rl11（t） mutant showed abnormal number and morphology of veins compared with those in wild type plants. In addition, rl11（t） mutant was less sensitive to the inhibitory effect of auxin than the wild type. Genetic analysis suggested that the mutant was controlled by a single recessive gene. Gene Rl11（t） was initially mapped between SSR markers RM6089 and RM124 on chromosome 4. Thirty-two new STS markers around the Rl11（t） region were developed for fine mapping. A physical map encompassing the Rl11（t） locus was constructed and the target gene was finally delimited to a 31.6 kb window between STS4-25 and STS4-26 on BAC AL606645. This provides useful information for cloning of Rl11（t） gene.

关 键 词：功能基因组学 精细定位 遗传分析 突变体 ORYZA 水稻 动叶 叶片形状 

分 类 号：S511[农业科学—作物学] Q78[生物学—分子生物学]