Effects of sympathetic histamine on vasomotor responses of blood vessels in rabbit ear to electrical stimulation  

交感神经源性组胺释放对电刺激条件下兔耳血管舒缩功能的影响(英文)

作　　者：陈瑛瑛[1] 吕军[1] 薛小燕[1] 何功浩[1] 周颖[1] 贾敏[1] 罗晓星[1] 

机构地区：[1]第四军医大学药学系药理教研室,西安710032

出　　处：《Neuroscience Bulletin》2010年第3期219-224,共6页神经科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.30770669,30800310)

摘　　要：Objective To investigate the effects of histamine receptor antagonists on vasoconstriction induced by electrical stimulation （ES） on posterior auricular nerve,and to explore the pre-and post-synaptic effects of sympathetic histamine on the vasomotor responses of vascular smooth muscle in rabbit ear.Methods ES was applied to posterior auricular nerves of the whole rabbit ear at 10 Hz,20 Hz and 40 Hz,respectively.Besides,the whole ear was perfused with different histamine receptor antagonists under constant perfusion pressure,and the changes in the flow rate of perfusate were observed.Results The flow rate of venous outflow was decreased by ES at all the 3 frequencies.The ES-induced vasoconstriction at 20 Hz and 40 Hz could be partly inhibited by H1 receptor antagonist chlorpheniramine （P0.05） .After exhaustion of histamine in mast cells by pretreatment with specific mast cell degranulator compound 48/80,chlorpheniramine could still inhibit the ES-induced flow rate reduction.In contrast,H2 receptor antagonist cimetidine could enhance the 40-Hz ES-induced flow rate reduction （P 0.05） .Moreover,ES-induced vasoconstriction at the 3 frequencies could all be enhanced by H3 receptor antagonist thioperamide （P0.05） .Conclusion Stimulation on the auricular nerve may evoke histamine release from sympathetic nerves rather than from mast cells.Moreover,the functions of sympathetic histamine vary from pre-synaptic modulation to post-synaptic vasoconstriction or vasodilatation,via activation of different histamine receptors.目的研究不同的组胺受体拮抗剂对电刺激耳后神经引起的血管收缩的影响,并探讨交感组胺对兔耳血管平滑肌舒缩功能的突触前及突触后效应。方法使用离体灌流的完整兔耳,研究不同频率的交感神经刺激引起的组胺释放对血管舒缩功能的影响。对耳后神经分别给予10Hz、20Hz和40Hz的电刺激,并在恒压灌流条件下,观察不同的组胺受体拮抗剂对灌流液流速的影响。结果三种频率的电刺激均能引起恒压灌流兔耳的静脉流出液流速降低。H1受体的拮抗剂氯苯那敏能部分拮抗20Hz及40Hz电刺激引起的流速降低效应(P<0.05)。应用肥大细胞脱颗粒剂化合物48/80耗竭组织肥大细胞中的组胺后,H1受体拮抗剂仍能抑制电刺激引起的流速降低。相反,H2受体拮抗剂西咪替丁能增强电刺激引起的流速降低效应,且只针对更高的刺激频率(40Hz,P<0.05)。此外,在H3受体拮抗剂噻普酰胺的作用下,三种频率的电刺激所引起的血管收缩都更为显著(P<0.05)。结论刺激耳后神经有可能引起组胺释放,且这种释放来自交感神经而非肥大细胞。通过刺激不同的组胺受体,交感组胺分别介导突触前调节和突触后血管收缩或舒张效应。

关 键 词：sympathetic nervous system HISTAMINE histamine receptors blood vessels 

分 类 号：R764[医药卫生—耳鼻咽喉科]