8-溴-环磷酸腺苷对单核细胞株THP1中ABCA1及细胞间黏附分子的影响  

Effects of 8-Br-cAMP on ABCA1 and ICAM-1 in THP-1 cells

作　　者：李建华[1] 杨永红[1] 贾军宏[1] 吴平生[2] 郭志刚[2] 

机构地区：[1]解放军总医院第一附属医院,北京100048 [2]南方医科大学南方医院心内科

出　　处：《中国老年学杂志》2010年第10期1378-1380,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(30171028);广东省自然科学基金(010616)

摘　　要：目的观察8-Br-cAMP刺激下,单核细胞株THP1中ABCA1、细胞间黏附分子-1(ICAM-1)及白介素-1β(IL-1β)mRNA和蛋白质表达的变化,阐明ABCA1基因在动脉粥样硬化(AS)形成中的可能机制。方法复苏培养THP1单核细胞,加入8-Br-cAMP(0.5mmol/L)刺激3、6、12、24h,设未加刺激同时孵育24h的细胞为阴性对照组;以荧光定量RT-PCR和Western印迹法及ELISA法检测ABCA1、ICAM-1及IL-1βmRNA和蛋白质表达量;用ABCA1的反义寡核苷酸(100nmol/L)转染THP1细胞,给予8-Br-cAMP刺激,同样的方法观察上述指标的改变。结果予8-Br-cAMP刺激后,THP1细胞中ABCA1、ICAM-1mRNA和蛋白质及IL-1β蛋白质表达均增高,给予反义寡核苷酸转染后氧化低密度脂蛋白(Ox-LDL)刺激3、6h上述指标的mRNA表达降低(P<0.01),12、24h蛋白质表达降低(P<0.01)。结论 THP1单核细胞中,8-Br-cAMP激活ABCA1不仅可增加细胞内胆固醇外运,还具有增加炎症因子表达的作用,在AS的发生中发挥双重作用。Objective To investigate the effects of 8-Br-cAMP on ABCA1,ICAM-1,IL-1βin human monocytes THP1 after the treatment with 8-Br-cAMP.Methods 8-Br-cAMP（0.5 mmol/L）were added into culture media and THP1 cells were harvested at 3,6,12 and 24 hours.The mRNA and protein levels of ABCA1、ICAM-1 and IL-1βwere investigated by real-time fluorescent quantitative PCR,Western blot and ELISA methods.After phosphorothioate antisense oligonucleotides of ABCA1 mixture were add to culture media at a final concentration of 100 nmol/L.Results The mRNA and protein of ABCA1,ICAM-1 and IL-1βamount were increased after the incubated with 8-Br-cAMP.After transfection with antisense oligonucleotides of ABCA1,the expression of mRNA levels were decreased at 3 and 6 hours,and protein were decreased at 12 and 24 hours.Conclusions ABCA1 can contribute to atherosclerogenesis by increasing the expression of ICAM-1 induced by 8-Br-cAMP in THP1 cells.

关 键 词：THP1单核细胞 ABCA1 动脉粥样硬化 炎症因子 

分 类 号：R392.12[医药卫生—免疫学]