利用烟草瞬时表达系统检测高等植物基因的剪接加工  

Application of Tobacco Transient Expression System to Detect Gene Splicing in Higher Plants

作　　者：高少培[1] 牛向丽[2] 罗笛[1] 常丽娟[1] 喻旭[1] 李欲翔[1] 刘永胜[1,2] 

机构地区：[1]四川大学生命科学学院,生物资源与生态环境教育部重点实验室,成都610064 [2]重庆大学农学及生命科学研究院,重庆400030

出　　处：《生物化学与生物物理进展》2010年第6期662-670,共9页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(30970260;30971752;30825030);国家转基因专项课题资助项目(2009ZX08009-072B)~~

摘　　要：解析基因的剪接加工机制是了解植物形态建成、生长发育和逆境胁迫应答的重要环节.与动物相比,植物中相应的研究进展较为缓慢.利用农杆菌介导的烟草瞬时表达系统,分别对单子叶植物水稻BADH2和双子叶植物拟南芥GR7基因片段在烟草叶片中的转录后剪接加工进行分析.结果表明,一些重要剪接调控元件在植物中保守存在,而烟草瞬时表达系统可以作为研究高等植物剪接调控的重要工具,快捷灵敏地检测基因的剪接加工方式.To dissect the splicing mechanism is a critical step in understanding the plant morphogenesis, growth, development and responses to stresses. Compared to animals, the research on RNA splicing is progressing poorly in plants. The splicing patterns of gene fragments derived from monocot rice BADH2 and dicot Arabidopsis GR7 were detected by Agrobacterium-mediated tobacco transient expression system. The results indicated that the fundamental splicing regulatory elements are conserved in plants. The tobacco transient expression system could be used as an important tool for fast and sensitive detection of gene splicing regulation in higher plants.

关 键 词：RNA剪接 烟草瞬时表达系统 高等植物 

分 类 号：Q5[生物学—生物化学] Q7