一氧化氮、鸟苷酸环化酶在扩张型心肌病小鼠心肌内表达及其与心肌损害的关系  

Expression and association of NO and GC with myocardial damage in mice with dilated cardiomyopathy

作　　者：印芳颖[1] 翟淑波[2] 聂抒[2] 孙景辉[2] 韩燕燕[2] 

机构地区：[1]吉林大学中日联谊医院儿科,吉林长春130033 [2]吉林大学第一医院小儿心血管科,吉林长春130021

出　　处：《临床儿科杂志》2010年第6期576-579,共4页Journal of Clinical Pediatrics

基　　金：长春市科技计划项目(No.2006085)

摘　　要：目的探讨一氧化氮(NO)、鸟苷酸环化酶(GC)在扩张型心肌病小鼠心肌内的表达及其与心肌损害的关系。方法将4～5周龄Balb/c小鼠随机分成实验组和正常对照组;以柯萨奇病毒B3(CVB3)感染Balb/c小鼠建立扩张型心肌病小鼠模型;通过比色法检测NO的变化,RT-PCR法测定外周血GC mRNA,HE、VG染色观察心肌损害程度。结果①小鼠感染3个月后心脏收缩功能明显减退[LVEF(61.74±7.47)%比(91.59±1.38)%];6个月后心室壁活动度明显减弱,左室收缩功能进一步减退[6个月LVEF为(50.38±12.91)%),9个月(46.57±8.32)%],与3、1个月时比较,差异有统计学意义(P<0.05)。②实验组NO水平1个月(40.48±9.89)μmol/L、3个月(46.37±11.23)μmol/L、6个月(74.57±15.26)μmol/L逐渐升高,9个月(32.54±7.63)μmol/L有所下降,但与对照组(25.09±5.54)μmol/L比较差异均有统计学意义(P<0.01)。③实验组GC mRNA表达于CVB3感染1个月(0.575±0.081)、3个月(0.763±0.056)、6个月(0.835±0.044)逐渐升高,与对照组(0.441±0.028)比较差异有统计学意义(P<0.01),9个月(0.482±0.037)虽有所下降,但与对照组比较差异无统计学意义(P>0.05)。④实验组NO表达与心肌病理积分呈显著正相关(r=0.548,P<0.05),GC mRNA表达量与NO亦呈显著正相关(r=0.613,P<0.05)。结论扩张型心肌病小鼠的心肌内表达了NO、GC,与心肌损害成正比。Objective To examine the myocardial expressions of NO and guanylate cyclase（GC） in mice with dilated cardiomyopathy,and analyze the relationship between their expressions and myocardial damage.Methods One hundred 4-5 weeks Balb/c mice were randomly divided into two groups： normal control group and experiment group.Dilated cardiomyopathy model was established by subjecting Balb/c mice to Coxsackie virus B3（CVB3） infection.The heart tissues were examined at 1,3,6,9 months after CVB3 infection.Expression of NO was measured by colorimetry methods,GC mRNA was determined by RT-PCR,myocardial damage was assessed by HE and VG staining.Results（1） Left ventricular systolic function was significantly decreased at 3 months in experimental group（LVEF 61.74% ± 7.47% vs 91.59% ± 1.38% in controls）.Ventricular wall activity was significantly decreased,and left ventricular systolic function was further impaired at 6 and 9 months,the differences were significant compared with 1 month and 3 months time points（P〈0.05）.（2） The expressions of NO in experiment group were 40.48 ± 9.89 μmol/L,46.37 ± 11.23 μmol/L,74.57 ± 15.26 μmol/L,and 32.54 ± 7.63 μmol/L,respectively,at 1,3,6,9 months,the difference between two groups at each time point was significant（P〈0.01）.（3） The expressions of GC mRNA in experiment group were 0.575 ± 0.081,0.763 ± 0.056,0.835 ± 0.044,and 0.482 ± 0.037,respectively,at 1,3,6,9 months.The difference between two groups was significant at 1,3,6 months,and without significance at 9 months.（4） NO expression in the experiment group was significantly correlated with cardiac pathology score（r = 0.548,P〈0.05）.GC mRNA expression in experiment group significantly correlated with NO expression（r = 0.613,P〈0.05）.Conclusions Expressions of NO and GC were found in mice with dilated cardiomyopathy,and they were positively associated with the severity of myocardial injury.

关 键 词：一氧化氮 鸟苷酸环化酶 扩张型心肌病 

分 类 号：R725.4[医药卫生—儿科]