新生期大鼠反复惊厥后皮层微管相关蛋白1轻链3的表达及干预  

The expression of cortex microtubule-associated protein 1 light chain 3 and intervention effect of 3-methlyadenine in neonatal rat with recurrent seizures

作　　者：巩勇[1] 倪宏[1] 陶陆阳[2] 

机构地区：[1]苏州大学附属儿童医院儿科医学研究所,江苏苏州215003 [2]苏州大学衰老与神经疾病实验室,江苏苏州215003

出　　处：《临床儿科杂志》2010年第6期584-588,共5页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.30470555;30870808);江苏省自然科学基金青年科技创新人才项目(No.BK2007509);江苏省高校自然科学基金资助项目(No.07KJB320103)

摘　　要：目的研究新生期大鼠反复惊厥后皮层中微管相关蛋白1轻链3(LC-3)的表达及3甲基腺嘌呤(3-MA)对表达的调控作用。方法生后6 d的SD大鼠随机分成3组,每组24只,惊厥组每日吸入三氟乙醚诱导惊厥发作5次,每次间隔30 min,连续9 d;对照组同样操作但不吸入三氟乙醚;3-MA组惊厥前腹腔注射3-MA(总量2μl),同样方法吸入三氟乙醚诱导惊厥,方法同惊厥组。3组大鼠于最后一次惊厥后分别按1.5、3、6、24 h后断头取脑,采用蛋白质免疫印迹技术(Western blot)分别检测3组大鼠大脑皮层中LC-3的表达。结果惊厥组(1.5、3、6 h)LC-3的表达明显高于对照组(t=2.483,2.599,6.937,1.280均P<0.05),3-MA组LC-3的表达明显低于惊厥组(t=2.243,7.775,5.887,4.245均P<0.05)。而24 h惊厥组LC-3的表达并不明显高于对照组(t=1.280,P>0.05)。结论惊厥后自噬途径被激活;3-MA参与了自噬途径的调控。Objective To investigate dynamic expression of cortex microtubule-associated protein 1 light chain 3（LC-3） and intervention effect of autophagy/lysosome inhibitor 3-methlyadenine（3-MA） in neonatal rat with recurrent seizures.Methods Seventy-two 6-day-old SD rats were randomly divided into three groups（24 rats in each）： recurrent seizures group（RS group）,3-MA-treated group（3-MA group）,and control group.Rats in RS group were induced to seizures 5 times each day for 9 days with aspiration of flurothyl during the first 12 days of life.Rats in 3-MA group were induced to seizures with the same method,and were treated with intraperitoneal injection of 3-MA before seizure-induction each day（totally 2 μl）.The brains of the rats were taken at 1.5,3,6,and 24 h after the last convulsion.Western blot was employed to determine alternations in LC3 in cortex at different time points.Results LC-3 expression was significantly higher in RS group than that of the control group at 1.5,3,and 6 h（P〈0.05）,while the difference between two groups at 24 hours after seizure was not significant.LC-3 expression decreased significantly in 3-MA group compared with those in RS group at all time points（P〈0.01）.Conclusions Autophagy/lysosomal pathway were activated immediately after recurrent seizures as indicated by the elevated expression of LC3 in cortex.3-MA was involved in the regulation of autophagy/lysosomal pathway.

关 键 词：惊厥 新生大鼠 微管相关蛋白1轻链3 自噬 3甲基腺嘌呤 

分 类 号：R722.1[医药卫生—儿科]