用单克隆抗独特型抗体NP30检测血吸虫病短程抗体的研究  

Study on the Self short life Antibody Detection of Schistosoma Japonicum with Monoclonal Anti idiotypic Antibody NP30

作　　者：仇镇宁[1,2] 冯振卿[1,2] 朱昌亮 李芸茜[1,2] 管晓虹 

机构地区：[1]南京医科大学第一附属医院临床检验中心分子室 [2]南京医科大学寄生虫学教研室

出　　处：《南京医科大学学报（自然科学版）》1999年第1期4-6,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家"八五"攻关计划"血吸虫病免疫诊断"专题项目;江苏省卫生厅"日本血吸虫单克隆抗独特型抗体NP30

摘　　要：为证实用日本血吸虫单克隆抗独特型抗体ＮＰ３０制备的抗体检测试剂盒考核治疗效果的价值，采用双“抗原”夹心酶联免疫吸附试验，用ＮＰ３０替代抗原，检测血吸虫病抗体。结果：该试剂盒对急性感染者的检出率为１００％，慢性轻度感染者的检出率为６１％，特异性为１００％。急性感染和慢性感染的Ｙｏｕｄｅｎ指数（ｒ）分别为１．０和０．６１，慢性感染者与经典抗体检测法ＳＥＡ／ＥＬＩＳＡ（０．６０）的ｒ无差别。用此试剂盒检测血吸虫病治疗后１ａ的血清，阴转率达１００％，明显优于经典抗体检测法（４３．６％）。结论：ＮＰ３０检测的抗血吸虫抗体属短程抗体，该试剂盒具有与循环抗原检测相同的考核疗效价值。This investigation is designed to evaluate the value in comment on the treatment effects of the diagnostic kit made by monoclonal anti idiotypic antibody NP30 of Schistosom japonicum . The antibody of Schistosoma japonica was detected by the “antigen sandwiched” ELISA. The antigen was replaced by NP30. The results revealed that the sensitivity was 61% in the mild and chronic infection and 100% in the acute infection; and that the specificity was 100%. The Youden index of the chronic infection was 0.61. There is no difference with classical antibody detection (SEA/ELISA 0.60). The correction index of conversion to negative rate in patients cured one year later was 100%. It was superior to the classical antibody detection(43.6%). It is suggested that the antibody detected with NP30 is self short life antibody of schistosomiasis . This test kit has the value in comment on the treatment effects equal to that of circulating antigen detection.

关 键 词：血吸虫病 抗独特型 抗体 短程抗体 ELISA 诊断 

分 类 号：R532.210.4[医药卫生—内科学]