聚环氧乙烷对大鼠提睾肌微循环红细胞流速的影响  

Effect of polyethylene oxide on red blood cell velocity in rat cremaster microcirculation

作　　者：杜荣生[1] 查道刚[1] 周冰洁[1] 胡锋[1] 纪丽景[1] 吴爵非[1] 宾建平[1] 刘伊丽[1] 

机构地区：[1]南方医科大学南方医院心血管内科,广东广州510515

出　　处：《南方医科大学学报》2010年第5期960-962,965,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30870721)

摘　　要：目的应用大鼠在体提睾肌模型,研究长链高分子化合物聚环氧乙烷(PEO)对微循环血流速度的影响,初步探讨减阻剂改善微循环的可能机制。方法 Wistar雄性大鼠6只,于术前1天,采集自体红细胞用异硫氰酸荧光素标记后备用。成功建立在体提睾肌模型后经颈静脉注入荧光素标记的自体红细胞,通过荧光显微镜观察提睾肌微循环并全程录像。全部大鼠经静脉给药2次(10ppmPEO及生理盐水各1次,注射顺序根据随机化表决定),速度均为3.5ml/h。两种药物的注射时间及停药后观察时间均为20min。试验全程监测心率和动脉血压。红细胞流速用IPP6.0专业图像分析软件测定。结果与注入生理盐水相似,注射PEO对大鼠心率、血压均无明显影响(P>0.05);注射生理盐水前、后大鼠微循环红细胞流速无明显改变(496.44±170.91μm/svs515.22±172.77μm/s,P=0.648),但注射PEO后可见大鼠微循环血流中红细胞流速显著增加(498.7±182.89μm/svs773.54±308.27μm/s,P=0.012)。结论 PEO在不影响大鼠心率、血压情况下,可明显增强大鼠提睾肌微循环红细胞流速,提示PEO可能通过增加局部血流速度的途径改善组织灌注。objectives To investigate the drag-reducing effect of polyethylene oxide （PEO） on the velocity of red blood cells in rat cremaster microcirculation. Methods Blood samples were collected from 6 Wistar male rats （100-110 g） via the post-orbital venous plexus. The red blood cells were separated by centrifugation and labeled by fluorescinisothiocyate （FITC）. After successful establishment of cremaster model, the labeled red blood cells were injected into the jugular vein, and the microcirculation was observed and recorded under fluorescence microscope. The hemodynamic parameters and microcirculation video was recorded every 4 min since 4 min before PEO or normal saline injection. Both PEO （10 ppm） and normal saline was injected into the same rat in random sequence at a constant rate of 3.5 ml/h for 20 min followed by observation for another 20 min. The volecity of the labeled-red blood cells was determined by IPP 6.0 software. Results Compared with normal saline, PEO significantly increased the velocity of the red blood cells in the rat cremaster microcirculation （498.7±182.89 μm/s vs 773.54±308.27 μm/s, P=0.012）. No significant changes in the heart rate and arterial blood pressure were observed during the experiment （P=0.836, P=0.420）. Conclusion PEO at an extremely low concentration can significantly increase the velocity of the red blood cells in rat cremaster microcirculation and produces no significant impact on heart rate and arterial blood pressure.

关 键 词：减阻剂 聚环氧乙烷 提睾肌 微循环 红细胞 

分 类 号：R697[医药卫生—泌尿科学]