特布他林对肺泡Ⅰ型和Ⅱ型细胞钠水转运机制的影响  

Effect of terbutaline on sodium transport in alveolar type Ⅰ and type Ⅱ cells

作　　者：徐建光[1] 李涛平[1] 王沛[2] 申海燕[1] 

机构地区：[1]南方医科大学南方医院睡眠中心,广东广州510515 [2]解放军457医院呼吸内科,湖北武汉430012

出　　处：《南方医科大学学报》2010年第5期966-968,972,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30471720)

摘　　要：目的探讨特布他林对肺泡Ⅰ型细胞(ATⅠ)和Ⅱ型细胞(ATⅡ)上Na+转运机制的影响。方法分离ATⅡ,采用膜片钳全细胞记录模式,应用全细胞钠通道(ENaC)阻断剂amiloride和环核苷酸门控阳离子通道(CNG)阻断剂ZnCl2观察ATⅡENaC和CNG电流及特布他林对两者的影响。结果 ATⅡ全细胞电流主要为amiloride敏感电流和Zn2+敏感电流,两者比例无差别(P>0.05);特布他林可使amiloride敏感电流和Zn2+敏感电流明显增加(P<0.05),amiloride敏感电流所占比例约为Zn2+敏感电流的1.7倍(P<0.05)。结论急性分离ATⅡ上存在功能性ENaC和CNG,并且Na+转运以两者为主。特布他林增强肺水吸收主要通过增加ATⅠ和ATⅡ上ENaC和CNG通道的Na+转运实现。Objective To explore the effect of terbutaline on sodium transport in rat alveolar type I （ATI） and type II （ATII） cells of rats. Methods The whole cell currents were recorded from ATII cells isolated from rat lungs perfused with or without amiloride （inhibitor of epithelial sodium channel） and ZnCl2 （inhibitor of cyclic nucleotide-gated cation channel） in the whole cell recording mode using the patch-clamp technique. The effect of terbutaline on the currents was examined. Results The main currents recorded from ATII cells were amiloride-sensitive and Zn2＋-sensitive. The amiloride-sensitive and Zn2＋-sensitive current shared a similar proportion （P0.05）. Both currents could be significantly increased by terbutaline （P0.05）, and the proportion of amiloride-sensitive current was 1.7 times that of Zn2＋-sensitive current （P0.05）. Conclusion There are functional epithelial sodium channels （ENaC） and cyclic nucleotide-gated cation channels （CNG） on freshly isolated ATII cells, both serving as the main channels for sodium transport. Terbutaline increases the absorption of alveolar fluid primarily by increasing sodium transport of ENaC and CNG on ATI and AT II cells.

关 键 词：肺泡Ⅰ型细胞 肺泡Ⅱ型细胞 上皮钠通道 环核苷酸门控阳离子通道 特布他林 

分 类 号：R96[医药卫生—药理学]