C57BL/6小鼠与BALB/c小鼠对脓毒症反应的差异  

Differences in the response to sepsis between C57BL/6 and BALB/c mice

作　　者：张婕[1] 刘占国 罗宇维[1] 何懿[1] 顾冬生[1] 王明[1] 郑彦涛[1] 孙尔维[1] 

机构地区：[1]南方医科大学珠江医院器官移植科,广东广州510282

出　　处：《南方医科大学学报》2010年第5期973-975,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30872312);国家自然科学基金-广东省政府联合基金(重点项目)(U08323003)

摘　　要：目的探讨C57BL/6与BALB/c两种小鼠对脓毒症的反应能力。方法 C57BL/6小鼠和BALB/c小鼠各30只,分别随机分成假手术组与脓毒症组,每组15只。脓毒症组用盲肠结扎穿孔法(CLP)建立小鼠脓毒症模型。术后6h,每组随机选出5只,Bio-plex悬浮蛋白芯片系统检测其外周血IL-1β、IL-2、IL-4、IL-5、IL-10、GM-CSF、IFN-γ和TNF-α的浓度。剩余小鼠用于1周存活率分析。结果两品系小鼠假手术组1周存活率均为100%。C57BL/6小鼠脓毒症组1周存活率为10%,BALB/c小鼠脓毒症组存活率为50%。C57BL/6小鼠脓毒症组的存活率显著低于BALB/c小鼠脓毒症组(P<0.05)。与假手术组相比,C57BL/6小鼠脓毒症组分泌的IL-4、TNF-α和IL-10明显升高。而BALB/c小鼠脓毒症组分泌的8种细胞因子较假手术组均未见明显增加。结论 C57BL/6与BALB/c两种小鼠对脓毒症的反应能力有差异,C57BL/6小鼠反应更敏感。Objective To compare the responses to sepsis between C57BL/6 and BALB/c mice. Methods Thirty C57BL/6 mice and 30 BALB/c mice were randomized into sham-operated group and sepsis group （n=15）. Sepsis model was established by cecal ligation puncture （CLP） in the mice, and 6 h after the operation, 5 mice from each group were selected randomly for cytokine detection including IL-1β, IL-2, IL-4, IL-5, IL-10, GM-CSF, IFN-γ and TNF-α by Bio-plex. The other 10 mice in each group were used for survival analysis. Results The survival rates of BALB/c and C57BL/6 mice were both 100% in one week after the sham operation, but lowered to 10% and 50% in one week after CLP, respectively. The survival rate of C57BL/6 mice was significantly lower than that of BALB/c mice （P0.05）. After CLP, C57BL/6 mice showed significantly greater IL-4, TNF-α and IL-10 production than the sham-operated mice, but the concentrations of the 8 cytokines in BALB/c mice after CLP showed no significant increment. Conclusion Compared with BALB/c mice, C57BL/6 strain mouse is more sensitive to sepsis.

关 键 词：C57BL/6小鼠 BALB/C小鼠 细胞因子 脓毒症 

分 类 号：R459.7[医药卫生—急诊医学]