厄贝沙坦对糖尿病大鼠早期肾脏组织结构影响的剂量相关性初探  

Dose-effect relationship of irbsartan with the changes in the renal tissue structure in diabetic rats

作　　者：唐杰龙[1] 沈洁[1] 刘帅[1] 范艳飞[1] 谢翠华[1] 

机构地区：[1]南方医科大学南方医院内分泌代谢科,广东广州510515

出　　处：《南方医科大学学报》2010年第5期1066-1068,1071,共4页Journal of Southern Medical University

基　　金：广东省科技计划项目(2007B010600010)

摘　　要：目的观察不同剂量厄贝沙坦对STZ诱导的糖尿病大鼠肾脏镜下组织结构影响的相关性,从组织学探讨厄贝沙坦剂量相关性的肾脏保护作用。方法 Wistar雄性大鼠链脲佐菌素(55mg/kg)造糖尿病大鼠模型,随机分成糖尿病对照组(D组)、25mg/kg厄贝沙坦组(I25组)、50mg/kg厄贝沙坦组(I50组)和200mg/kg厄贝沙坦组(I200组),同龄大鼠作为正常对照组(C组)。造模4周后采用灌胃法给药8周,计算肾脏质量指数;ELISA法检测24h尿白蛋白排泄率;图像分析软件测定肾小球体积。结果给药8周后,各药物干预组的24h尿白蛋白排泄率、肾脏质量指数、肾小球体积均显著降低,以I50和I200组降低最为明显。结论在一定剂量范围内,厄贝沙坦对糖尿病大鼠的肾脏组织结构的保护作用呈剂量依赖性。Objective To investigate the relationship between the dosage of irbesartan and the renal tissue structure in diabetic rats. Methods Male Wistar rats was given a single intraperitoneal dose （mg/kg） of streptozotocin to induce diabetes. The diabetic rats were randomized into 4 groups and received 4 weeks later 25 mg/kg （n=9）, 50 mg/kg （n=9）, 200 mg/kg （n=9） irbesartan intragastrically, or equal volume of water （model group, n=11） on a daily basis. Seven normal rats receiving with equal volume of water served as the normal control. All the rats were sacrificed after 8 weeks and the 24-hour albumin excretion, renal mass index and the volume of the glomerulus were measured. Results The 24-hour albumin excretion, renal mass index and volume of the glomerulus in the 3 irbesartan groups were significantly decreased as compare with those in the model group; the reductions in 50 and 200 mg/kg irbesartan gourps were significant greater than those in 25 mg/kg irbesartan group. Conclusion Irbesartan can decrease the 24-hour urinay albumin excretion and relive glomerulopathy in diabetic rats. Within a certain dose range, irbesartan produces a dose-dependent protective effect on the renal structures in the diabetic rats.

关 键 词：糖尿病肾病 血管紧张素Ⅱ受体拮抗剂 厄贝沙坦 

分 类 号：R587.2[医药卫生—内分泌]