BAFF启动子-871C/T基因多态性在ITP患者中的意义  

Significance of B Cell Activating Factor of TNF Family Promoter Polymorphisms in Patients with Immune Thrombocytopenic Purpura

作　　者：刘俊庆[1] 杨林花[1] 陈秀花[1] 覃艳红[1] 刘秀娥[1] 陈剑芳[1] 常丽贤[1] 高艳[1] 

机构地区：[1]山西医科大学第二医院血液科,山西太原030001

出　　处：《中国实验血液学杂志》2010年第3期690-693,共4页Journal of Experimental Hematology

基　　金：2008年国家公益性科研专项基金;编号200802031

摘　　要：本研究通过检测B细胞活化因子(BAFF)启动子-871C/T基因多态性在特发性血小板减少性紫癜(ITP)患者中的分布情况,探讨其与ITP发病的相关性,并研究ITP患者初诊时血小板计数与BAFF-871C/T基因型的关系。选择133例ITP患者和117例健康对照者,应用等位基因特异性PCR(ASP?PCR)及琼脂糖凝胶电泳检测BAFF-871C/T多态性,判定各研究对象的基因型,并统计各基因型及等位基因的频率。结果表明:C/C、C/T、T/T3种基因型分布在ITP组分别为33.1%、42.1%、24.8%,对照组分别为55.6%、33.3%、11.1%。T等位基因频率在ITP组为45.9%、对照组为27.4%,差异均有统计学意义(p<0.05)。ITP组T等位基因频率高于对照组。各基因型间血小板计数无统计学差异(p>0.05)。结论:BAFF启动子-871C/T多态性可能与ITP的发病有相关性,但各基因型之间患者初诊时血小板计数没有差别,不能作为病情严重程度的评价指标。The study was aimed to examine the B cell activating factor promoter polymorphism of the TNF family （BAFF）-871 C/T in patients with immune thrombocytopenic purpura （ITP） and to explore its correlation with ITP and the relationship between the blood platelet count of newly diagnosed patients with ITP and genotypes of BAFF -871 C/T polymorphisms. Alleles specific polymerase chain reaction （ASP-PCR） and agarose gel electrophoresis were used to identify polymorphisms -871 C/T of BAFF promotor in 133 ITP patients and 117 healthy controls, and determine the genotype of subjects. Meantime, the frequency of genotype and alleles were analyzed. The results indicated that out of 133 patients with ITP, 33.1% patients exhibited C/C, 42.1% patients were heterozygous C/T, and 24.8% patients were homozygous T/T. The corresponding frequencies in 117 healthy controls were 55.6% C/C, 33.3% C/T and 11. 1% T/T. The allele frequency of T in ITP patients and healthy controls were 45.9% and 27.4% respectively. There was significant difference in the BAFF-871 C/T genotypic frequency between the ITP patients and healthy controls（p 〈 0.05）. The allele frequency of T in ITP patients was higher than that in healthy controls. There was no significant difference in the blood platelet counts between the various genotype （p 〉 0.05 ）. It is concluded that the polymorphism - 871 C/T of BAFF promoter is correlated with the pathogenesis of ITP. However, there is no significant difference in blood platelet counts between the various genotype, so the polymorphism -871 C/T of BAFF promoter can not be referred as the analysis index for evaluating the severity of ITP.

关 键 词：特发性血小板减少性紫癜 B细胞活化因子 基因多态性 

分 类 号：R554.6[医药卫生—血液循环系统疾病]