Notch配体Delta-like 1对小鼠骨髓细胞来源的树突状细胞分化和抗原呈递功能的影响  

Role of Delta-like 1 in Differentiation and Antigen Presentation of Mouse Bone Marrow-Derived Dendritic Cells

作　　者：陈韵如[1] 丰帆[2] 尹郸丹[1] 梁英民[1] 韩骅[3] 

机构地区：[1]第四军医大学唐都医院血液内科,陕西西安710038 [2]第四军医大学西京医院消化外科,陕西西安710032 [3]第四军医大学医学遗传学与发育生物学教研室,陕西西安710032

出　　处：《中国实验血液学杂志》2010年第3期704-708,共5页Journal of Experimental Hematology

基　　金：国家高技术研究计划课题,编号2006AA02A111

摘　　要：本研究探讨Notch配体Delta-like 1(Dll1)对小鼠骨髓细胞来源的树突状细胞(dendritic cell,DC)分化及其抗原呈递功能的影响。在GM-CSF和IL-4存在条件下,用OP9-Dll1和OP9-GFP细胞分别与小鼠骨髓细胞共培养8天,经肿瘤抗原刺激成熟。用流式细胞仪检测DC表面MHCII、CD80和CD86的表达情况,ELISA法检测肿瘤抗原刺激后DC培养上清细胞因子IL-12和IL-10的水平,通过混合T淋巴细胞反应观察DC对T细胞的促增殖能力。结果表明:与GFP组相比,Dll1组的小鼠骨髓来源的树突状细胞明显增多(p<0.05)。肿瘤抗原刺激后,Dll1组DC表面MHCII、CD80和CD86表达量更高。DC分泌的IL-12水平显著高于对照组(p<0.05),而IL-10的水平显著低于对照组(p<0.01)。Dll1组DC具有更强的促T细胞增殖活性。结论:OP9-Dll1促进小鼠骨髓细胞向DC分化并增强其抗原呈递功能。The aim of this study was to investigate the role of Delta-like 1 （ Dill ） in diffrentiation and antigen presentation of mouse bone marrow-derived dendritic cells （DCs）. In the presence of granulocyte macrophage colony stimulating factor （GM-CSF） and interleukin 4 （1L-4）, mouse bone marrow cells were co-cultured with OP9-Dlll and OP9- GFP cell lines respectively. After 8 days, the immature DCs were stimulated with tumor antigen. The surface molecules of the activated DCs including MHC II, CD80 and CD86 were analyzed by flow cytometry. Levels of IL-12 and IL-10 in the culture supernatant were detected by ELISA. In addition, the proliferation of T-cells co-cultured with DCs was analyzed by FACS through mixed T-lymphocyte reaction. The results showed that compared with OP9-GFP, the bone marrow cells co-cultured with OP9-Dlll produced significantly more CD11 c ^＋ DCs （p 〈 0.05 ）, and possessed higher levels of surface molecule expression including MHC II, CD80 and CD86 after tumor antigen stimulation. The DCs secreted higher level of IL-12（p 〈0.05） and less IL-10 （p 〈0.01）. They also resulted in significantly stronger T-cell proliferation response. It is concluded that Dill can promote the differentiation of DCs from mouse bone marrow ceils and enhance their antigen presentation capacity.

关 键 词：树突状细胞 NOTCH Delta-like 1 T细胞 骨髓细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R730.3[医药卫生—基础医学]