漆酶/木聚糖酶体系预处理促进马尾松硫酸盐浆漂白的研究  

Study on Laccase/xylanase System Pretreatment in Pinus Massoniana Kraft Pulp Bleaching

作　　者：吴淑芳[1] 尤纪雪[1] 叶汉玲[1] 张琳[1] 

机构地区：[1]南京林业大学江苏省制浆造纸科学与技术重点实验室,江苏南京210037

出　　处：《中国造纸》2010年第6期10-13,共4页China Pulp & Paper

基　　金：国家自然科学基金资助项目;编号3067154

摘　　要：调控培养白腐菌Flammulina velutipes得到漆酶/木聚糖酶体系LXS(laccase-xylanase),将LXS用于马尾松硫酸盐浆(KP)漂白预处理研究,得到优选的预处理条件为:pH值4.2、浆浓3%、温度40℃、预处理时间3h和酶用量10IU/g。经LXS和漆酶介体体系LMS(laccase-mediator)预处理后的浆料再经碱抽提,卡伯值分别比对照浆降低9.2%和8.3%。LXS预处理后的浆料再经DED漂白,漂后浆料白度比对照浆高6.0%,接近经LMS预处理再漂白的浆料白度,并且经上述两种漆酶体系预处理的漂白浆的强度与对照浆相比均没有明显降低。漆酶/木聚糖酶体系有望代替昂贵的漆酶/介体体系实现漆酶用于纸浆漂白的工业化生产。LXS （laccase/xylanase system） was obtained after regulated cultivation of Flammulina velutipes. Pinus massonia kraft pulp was pretreated with LXS before DED sequence bleaching and the result were compared with the LMS （laccase/mediator system） pretreatment. The result showed that the optimal conditions of pretreatment were as following： pH 4.2, pulp consistency 3%, temperature 40℃, treatment time 3 h, and enzyme dosage 10 IU/g. The kappa numbers of the pulps which were pretreated with LXS and LMS and then extracted with alkali were reduced 9.2% and 8.3% compared with the control sample respectively. The brightness of the pulp pretreated with LXS and then bleached with DED sequence was close to that of the bleached pulp pretreated with LMS, and was 6.0% higher than that of the control pulp. Moreover, the strength of these two bleached pulps pretreated with LXS or LMS systems was similar and there was no obvious decrease compared with that of the control pulp.

关 键 词：漆酶 漆酶/介体体系 漆酶/木聚糖酶体系 漂白 马尾松 

分 类 号：TS745[轻工技术与工程—制浆造纸工程] Q55[生物学—生物化学]