预处理对废纸纤维素酶水解的影响  

Effects of pretreatment on enzymatic hydrolysis of cellulose for waste paper

作　　者：邓立红[1] 肖领平[1] 唐勇[1] 宋先亮[1] 

机构地区：[1]北京林业大学材料科学与技术学院

出　　处：《北京林业大学学报》2010年第3期170-175,共6页Journal of Beijing Forestry University

基　　金：北京林业大学新进教师科研启动基金项目(BLX2008011)

摘　　要：为了促进废纸类生物质资源的回收利用,采用蒸汽爆破、NaClO2处理、NaClO2结合NaOH处理、NaOH处理和氨水浸泡这5种方法对废鞋盒碎片进行预处理,以改善废纸的纤维素酶水解。分析了预处理后废纸中木素和半纤维素含量、纤维的表面结构和结晶度的变化,并探讨了这些变化对纤维素酶水解效果的影响。结果表明:蒸汽爆破预处理对木素和半纤维素的去除效果最好,碱处理对半纤维素的去除效果不好。预处理使原料的表面变得粗糙,尤其是NaOH处理导致的变化最大。预处理后,原料的结晶度有升有降。酶解试验表明木素和半纤维素的含量、原料表面粗糙程度与纤维素酶水解速率之间均不存在明显的相关性。但是结晶度与纤维素酶水解速率关系明显,结晶度越低,酶水解速率越快。NaOH预处理的原料结晶度最低,纤维素酶水解率最高,为45.4%。In order to accelerate the recovery and reuse of biomass resources,such as waste paper,different pretreatment methods were adopted to pretreat shredded shoe boxes as a model of waste paper to enhance the enzymatic hydrolysis of cellulose.These pretreatment were steam explosion,NaClO2 pretreatment,a pretreatment combination of NaClO2 and NaOH,NaOH pretreatment and aqueous ammonia steeping.We investigated the modification of the concentration of lignin and hemicellulose,surface structure and degree of crystallinity of the materials after pretreatments and discussed the effects of these modifications on the enzymatic hydrolysis of cellulose.Our experimental results indicate that steam explosion is the most efficient method in the removal of lignin and hemicellulose,while the alkali pretreatment exhibits a weak capacity in the removal of hemicellulose.The surface of the pretreated material became coarser,especially for NaOH pretreated samples.The pretreatments also resulted in an increase or decrease in the degree of crystallinity of the material.The results of enzymatic hydrolysis revealed no significant correlation between the rate of enzymatic hydrolysis and concentrations of lignin and hemicellulose,nor with the degree of coarseness of the material surface.But we found a significant correlation between the degree of crystallinity and the rate of enzymatic hydrolysis：the lower the degree of crystallinity,the faster the rate of hydrolysis.The degree of crystallinity was lowest in the NaOH pretreated material,with the highest rate of cellulose hydrolysis,i.e.,45.4%.

关 键 词：预处理 木素 半纤维素 表面结构特征 结晶度 

分 类 号：TQ9[化学工程]