当归补血汤对大鼠局灶性脑缺血再灌注后大脑皮质神经元的保护作用  

Protective effect of the decoction of angelicae sinensis for enriching blood on the cerebral cortex neurons of the focal cerebral ischemia reperfusion rats

作　　者：罗亚非[1] 石献忠[2] 胡琴[2] 孟芝君[3] 陆莹[1] 周长满[2] 

机构地区：[1]贵阳中医学院解剖学教研室,贵阳550002 [2]北京大学医学部解剖学与组织胚胎学系,北京100191 [3]北京大学医学部细胞生物学系,北京100191

出　　处：《解剖学报》2010年第3期367-373,共7页Acta Anatomica Sinica

摘　　要：目的探讨大鼠局灶性脑缺血再灌注后当归补血汤对大脑皮质神经元的保护作用。方法雄性SD大鼠48只随机分为假手术组、大脑中动脉阻塞再灌注组、生理盐水对照组、当归补血汤治疗组。治疗组术前连续6d灌胃,每日2次(早、晚各1次),术后4h再灌胃2次,每次2.5ml,次日取材。观察各组大鼠神经行为学缺陷;脑梗死体积和梗死率变化;血脑屏障通透性变化;大脑皮质神经元的形态和数量;大脑皮质神经元p53和天冬氨酸特异性半胱氨酸蛋白酶3(Caspase-3)的表达情况以及大脑皮质凋亡神经元的形态和数量。结果与缺血再灌注组、生理盐水对照组比较,当归补血汤治疗组2,3,5-氯化三苯四氮唑(TTC)染色显示大脑皮质梗死区体积减少(P<0.05);荧光显微镜观察脑组织中伊文斯蓝的含量减少(P<0.05),Nissl染色显示大脑皮质神经元数量增加(P<0.05);免疫组织化学方法显示大脑皮质神经元Caspase-3、p53的阳性细胞数减少(P<0.05);TUNEL染色法显示大脑皮质神经元凋亡细胞减少(P<0.05)。结论当归补血汤治疗组能减少大脑皮质神经元的死亡,这与其减少大脑皮质神经元Caspase-3、p53的表达,并通过减少细胞凋亡起保护作用有关。Objective To investigate the protective effect of the cerebral cortex neurons with the decoction of angelicae sinensis for enriching blood on the focal cerebral ischemia-reperfusion rats. Methods Forty-eight male SD rats were randomly divided into sham operation group, middle cerebral artery occlusion and reperfusion group, normal saline control group, decoction-treatment group. Before being operated, treatment-group rats were lavaged for 6 days, twice a day （taking medicine once in the morning and once in the evening ）. Four hours after being operated, the rats were lavaged twice each 2.5ml. Rats will be sacrificed the following day. The defects of neurological behavior of the rats and the changes of cerebral infarct volume with TTC staining were also observed; 2% the Evans blue was injected into the femoral vein of rats to observe permeability changes of blood-brain barrier; The numbers and morphological change of neurons in the cerebral cortex were counted through Nissl staining; The p53 and Caspase-3 expression of the neurons in the cerebral cortex were detected with immunohistochemical method; Meanwhile, The apoptotic neurons in the cerebral cortex were observed through terminal deoxynucleotidyl transferase mediated dUTP nick end labelling （TUNEL） staining. Results When compared with the middle cerebral artery occlusion/reperfusion （MCAO） and control saline group, the infarct size in the decoction-treatment group was markedly smaller than that observed in TTC-staining （P 〈 0. 05）. When brain slices were observed under a fluorescence microscope, less EB spillover was observed in the decoction-treatment group （P 〈 0. 05 ）. The number of neurons in the cerebral cortex increased as observed by Nissl-staining（ P 〈 0.05 ）. The number of positive immunohistochemistry staining of Caspase-3 and p53 was reduced （ P 〈 0. 05 ）. The number of apoptosis cell in the cerebral cortex was also reduced. （P 〈 0. 05）. Conclusion The decoction-treatment group could reduce death of neuron

关 键 词：局灶性脑缺血再灌注 当归补血汤 免疫组织化学 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]