表没食子儿茶素-3-没食子酸酯抑制小鼠皮肤外伤愈合组织基质金属蛋白酶1的表达  

Epigallocatechin-3-gallate inhibits the expression of matrix metalloproteinase-1 in mouse cutaneous wound healing

作　　者：申羽佳[1] 李卫国[1] 张江江[1] 张惠[1] 袁红旭[1] 贾永芳[1] 王坤英[1] 

机构地区：[1]河南师范大学生命科学学院动物学系,河南新乡453002

出　　处：《解剖学报》2010年第3期395-399,共5页Acta Anatomica Sinica

基　　金：河南省科技攻关计划(0624410029);河南省教育厅自然科学基础研究计划(2010A180011);新乡市科技发展计划项目(08S044);河南省重点学科基金

摘　　要：目的探讨50 mg/L表没食子儿茶素-3-没食子酸酯(EGCG)对小鼠皮肤外伤愈合组织基质金属蛋白酶1(MMP-1)mRNA表达及MMP-1水平的影响。方法将57只小鼠随机分为对照组(含无损伤组和损伤对照组)、50 mg/L EGCG处理组和辅料组,除无损伤组为3只外,其余各组均为18只,然后分别于损伤后6、12、24、72、120、168 h取损伤愈合皮肤。利用RT-PCR和酶联免疫吸附法(ELISA)检测皮肤外伤愈合期间损伤愈合组织MMP-1 mRNA表达水平和MMP-1含量。结果检测结果表明,50 mg/L EGCG能在皮肤外伤愈合晚期(72 h后)抑制损伤愈合组织MMP-1 mRNA表达,降低MMP-1含量。结论 EGCG对皮肤外伤愈合、晚期愈合组织MMP-1mRNA表达和MMP-1生成具有抑制作用,提示50 mg/L的EGCG能促进皮肤外伤愈合。Objective To explore the effects of epigallocatechin-3-gallate（EGCG） （50 mg/L） on the expression of MMP-1 mRNA and the levels of MMP-1 during mouse cutaneous wound healing. Methods Fifty-seven mice were divided into control, 50 mg/L EGCG and vehicle groups. The wound skins were prepared at 6, 12, 24, 72, 120 and 168 hours after injury, respectively. The expression of MMP-1 mRNA and levels of MMP-1 in wound healing skins were determined with reverse transcription polymerase chain reaction （RT-PCR） and enzyme-linked immunosorbent assay （ELISA）. Results It was found that 50 mg/L EGCG inhibits the expression of MMP-1 mRNA and decreases the levels of MMP-1 of the wound tissue at the late stage of wound healing （72 hours after skin injury）. Conclusion 50 mg/L EGCG could inhibit the expression of MMP-1 mRNA and the MMP-1 production during cutaneous wound healing, and promote skin recovering.

关 键 词：表没食子儿茶素-3-没食子酸酯 基质金属蛋白酶-1 愈合 皮肤 反转录-聚合酶链式反应 酶联免疫吸附法 小鼠 

分 类 号：Q249[生物学—细胞生物学] Q786