东北虎幼体血清酶含量的测定与分析  

Determination and analysis of eight sero-enzymes' biochemical indexes of panthera tigris amureusis

作　　者：张红绪[1] 李林[1] 刘丹丹[1] 王莉[1] 曹静[1] 段如冰[1] 

机构地区：[1]河南师范大学生命科学学院动物学教研室,河南新乡453007

出　　处：《解剖学报》2010年第3期405-408,共4页Acta Anatomica Sinica

基　　金：河南省自然科学基金资助项目(0611033200);河南省动物学省级重点学科经费资助

摘　　要：目的测定东北虎幼体血液中8种血清酶含量,为东北虎的生长发育、繁殖及疾病诊治等积累数据,并为虎类亚种间遗传和进化关系等的研究提供参考。方法用HITCH 7600-20型全自动生化分析仪对13只东北虎幼体进行8种血清酶含量的测定。结果东北虎幼体血清中8种酶含量分别为:谷草转氨酶(AST)(29.31±5.88)IU/L,乳酸脱氢酶(LDH)(267.08±76.40)IU/L,羟丁酸脱氢酶(α-HBDH)(149.38±54.07)IU/L,谷酰转肽酶(GGT)(2.92±1.94)IU/L,肌酸激酶(CK)(284.77±132.02)IU/L,淀粉酶(AMY)(2 149.85±357.03)IU/L,肌酸激酶同工酶(CK-MB)(390.62±145.70)IU/L,乳酸脱氢酶同工酶(LDI)(11.11±7.08)IU/L。结论东北虎幼体雌雄个体间所测8种血清酶含量无显著差异,与同科动物猎豹、金钱豹比较在AST、LDH等酶类存在差异。Objective The eight enzymes＇ levels in serum from Panthera tigris amureusis（P, t. amureusis） were determined. The data wouldl be accumulated for the growth, reproduction and disease diagnosis and treatment of young P. t. amureusis, as well as types of tiger subspecies genetic and evolutional relationship studies. Methods Eight enzymes＇ biological indexes of 13 individuals had been measured and analyzed in P. t. amureusis by HITCH 7200-20 automated chemistry analyzer, respectively between male and female young P. t. amureusis, and between young P. t. amureusis and adult. Results Aspartate aminotransferase（AST） was （29.31 ±5.88）IU/L, lactate dehydrogenase enzyme（LDH） was （267.08 ± 76. 40 ） IU/L, α-hydroxybutyrate dehydrogenase （ α-HBDH ） was （ 149.38 ± 54.07 ） IU/L, gamma-glutamyl transpeptidase（GGT） was （2.92 ± 1.94） IU/L, creatine kinase （CK） was （284.77 ± 132.02 ） IU/L, creatine kinase isoenzyme（CKMB） was （390.62 ± 145.70）IU/L and actate dehydrogenase isoenzyme（LDI） was （11.11 ±7.08）IU/L. Conclusion There are no differences in values of eight enzymes between male and female P. t. amureusis,but some differences such as AST and LDH are existed among P. t. amureusis ,young Acinonyx jubatus and young Chinese leopard in Felidae.

关 键 词：血液学 血清酶 连续监测法 东北虎幼体 

分 类 号：Q55[生物学—生物化学]