小鼠胚胎心脏发育期T型钙离子通道的表达  

Expression of T-type calcium channels during mouse embryonic heart development

作　　者：钱辉[1] 张沙沙[1] 袁秀杰[1] 赵钟钟[1] 石洁[1] 郭佳[1] 刘金旭[1] 朱中华[1] 张朝[1] 

机构地区：[1]南京师范大学生命科学学院江苏省分子医学生物技术重点实验室,南京210046

出　　处：《解剖学报》2010年第3期469-472,共4页Acta Anatomica Sinica

基　　金：国家重点基础研究发展计划资助项目(2007104GJB0117)

摘　　要：目的检测哺乳动物心脏中T型钙离子通道α1亚基在小鼠胚胎心脏发育过程中表达部位和时间的分布特征。方法对小鼠胚胎心脏发育关键时期(E9.5至胚胎E18.5共9d)的胚胎进行连续切片,用RT-PCR和免疫组织化学的方法对α1G和α1H蛋白进行阳性检测。结果α1H从胚胎期10.5d左右开始表达并一直延续到发育晚期(E18.5),而α1G则从E11.5d延续到E18.5;免疫组织化学的结果显示,T型钙离子通道主要在心肌层以及左右传导束支稳定表达,而在心内膜垫以及心室肌小梁的心内膜内皮和间充质细胞中不表达。结论 T型钙离子通道对于心肌细胞以及心脏传导组织的形成起到了一定的作用。Objective To investigate the temporal and spatial expression pattern of the pore-forming α1 subunits of T-type calcium channels during mouse embryonic heart development. Methods Histology and immunohistochemistry of mouse embryonic hearts from embryonic 9.5 days （E9.5） to El8.5 in paraffin-embedded were used to analyze the expression of α1G and α1 H, two subtypes pore-forming α1 subunits of T-type calcium channels. Results α1H proteins were detectable from earlier embryonic days （E10.5） to late embryonic day （ E18.5 ） white α1G proteins from E11.5 to E18.5. Immunohistochemical localization showed that the expression of α1H or α1G throughout myocardium and bundle branches with exception of the endothelium and mesenehyme cells among the trabecular and endocardial cushion region. Conclusion T-type calcium channels play an important role in the morphogenesis of myocardium and cardiac conduction system during mouse embryonic heart development.

关 键 词：心脏 发育 T型钙离子通道 免疫组织化学 反转录-聚合酶链式反应 小鼠 

分 类 号：Q132.4[生物学—普通生物学] Q954.561