小脑移植的基础与临床研究  

Embryonic cerebellar cell grafts in basic research and clinical application

作　　者：吴承远[1] 王建刚[1] 杨扬[1] 鲍修风[1] 周茂德[1] 朱树干[1] 李新钢[1] 刘玉光[1] 徐淑军[1] 

机构地区：[1]山东医科大学附属医院

出　　处：《中华器官移植杂志》1999年第1期15-16,共2页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金

摘　　要：目的为了证实小脑组织移植后移植物在受体脑内是否存活以及植入的神经细胞是否可以纠正和改善小脑功能缺陷和症状。方法将胎龄为１３天的Ｃ５７ＢＬ／６小鼠胚胎小脑组织悬液移植到３个月龄的ＢＡＬＢ／ｃ成年小鼠脑内，术后观察１～８周，并进行组织学观察。在动物实验基础上，将胎龄为９～１２周的人胚胎小脑组织悬液移植到６例患有严重小脑萎缩的患者脑内。结果动物实验的组织切片证实移植物在受体脑内再生，并且可见浦氏细胞分化成熟和迁徙到颗粒细胞层。６例患者接受小脑移植后近期疗效良好，症状和体征及客观指标均有改善。经过５～７年随访，仅２例患者疗效稳定。结论未成熟小脑组织移植后可在受体脑内存活，并且有神经细胞再生和改善神经功能潜力，但术后的排斥反应尚需深入研究。Objective To verify whether the cerebellar cell grafts to be survival in the recipient brain and the transplanted neurons to correct and improve the cerebellar function disorders and symptoms so as to explore a new way to treat cerebellar lesions. Methods Embryonic cerebellar tissue suspension from 13 day C57BL/6 mice was transplanted into the injured cerebellum of 3 month BALB/c mice. After operation, observation(including histological observation)was conducted for 1 to 8 weeks. On the basis of animal experiment, 9～12 week human embryonic cerebellar tissue suspension was transplanted into the cerebellum of 6 medically intractable patients with severe hereditary cerebellar degenerative ataxia.Results The histological observation of the cerebellar tissue in the animal experiment revealed that embryonic grafts could survive and that mitoses of the host Purkinje cells of the implanted group were increased substantially over the control groups. The short term therapeutic effects were good in the 6 patients following cerebellar transplantation. The symptoms, signs, and objective parameters were all improved to some extent. After 5 to 7 years' follow up, only 2 patients had a good result.Conclusion The embryonic cerebellar tissue can survive in the recipient cerebellum and has the potential to regenerate the neurons and improve the nervous function.

关 键 词：小脑移植 移植物 存活 排斥反应 

分 类 号：R651.11[医药卫生—外科学]