微囊化大鼠胰岛异种移植治疗小鼠实验性糖尿病的研究  

Xenotransplantation of microencapsulated rat islets to treat experimental diabetes in mice

作　　者：周茂华[1,2,3,4,5] 陈东明[1,2,3,4,5] 夏兆骥 丁亮华[1,2,3,4,5] 贾铁利 李静平[1,2,3,4,5] 王传民 朱洪荫[1,2,3,4,5] 

机构地区：[1]天津武装警察部队医学院外科教研室 [2]北京医科大学第三临床医学院成形外科研究中心 [3]齐齐哈尔医学院 [4]包头医学院 [5]空军总医院

出　　处：《中华器官移植杂志》1999年第1期17-19,共3页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金

摘　　要：目的研究海藻酸钠聚赖氨酸海藻酸钠包裹胰岛进行移植的效果。方法将Ｗｉｓｔａｒ大鼠的胰腺先行胶原酶胰管内注射消化，然后分离、纯化，所得胰岛经培养后制成微囊包膜的胰岛，微囊直径为０．４～０．５ｍｍ，每个微囊内包含１个胰岛。将微囊包膜及非包膜的胰岛移植至小鼠腹腔内，每个受体的移植量约１０００个胰岛。术后不用免疫抑制剂。结果微囊化的胰岛分别置含葡萄糖５．６ｍｍｏｌ／Ｌ和１６．７ｍｍｏｌ／Ｌ的无血清ＴＣ１９９培养基中培养，其胰岛素的释放量，包膜组与非包膜组相比，差异不显著（Ｐ＞０．０５）。微囊化胰岛移植组术后３天非空腹血糖从（２２．０±０．５１）ｍｍｏｌ／Ｌ降至（７．８±０．４８）ｍｍｏｌ／Ｌ，维持正常血糖的时间平均为１２６天；单纯胰岛移植组术后移植物有功能存活不足８天。结论微囊化胰岛移植后可在不使用免疫抑制剂的情况下延长胰岛移植物的存活时间。Objective To study the effects of alginate polylysine alginate(APA) microencapsulated islet in xenotransplantation. Methods Islets were isolated from Wistar rat pancreas by ductal injection of collagenase, digested, and purified by using Ficoll gradient. After cultured, the islets were encapsulated in PAP semipermeable membranes. The microencapsule had a diameter ranging from 0.4 to 0.6 mm with each microencapsule containing one islet. 1 000 encapsulated or non encapsulated islets were intraperitoneally transplanted into the diabetic mice in the absence of immunosuppression. Results There was no significant difference in insulin secretion between the encapsulated islets and non encapsulated ones ( P > 0.05 ). Diabetes was reversed within 3 days after transplantation of encapsulated islets with the fasting plasma glucose levels being dropped from ( 22.0 ± 0.51 )mmol/L to ( 7.8 ± 0.48 )mmol/L and maintaining a mean normoglycemia for 126 days. While the non encapsulated islets only functioned less than 8 days posttransplantation. Conclusion The encapsulated pancreatic islets, semipermeable membranes, can effectively prolong the xenograft survival without immunosuppression, and reverse hyperglycemia in diabetic mice.

关 键 词：胰岛移植 糖尿病 免疫学 异体移植 实验研究 

分 类 号：R587.105[医药卫生—内分泌] R657.5[医药卫生—内科学]