补肾益气活血方对胎儿生长受限孕鼠胎盘滋养细胞凋亡的影响  

Effect of Bushen Yiqi Huoxue Recipe on Placental Trophoblast Apoptosis in Fetal Growth Restricted Pregnant Rat

作　　者：陈镇燕[1] 王琪[1] 黄光英[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030

出　　处：《中国中西医结合杂志》2010年第6期611-616,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.39870923)

摘　　要：目的观察补肾益气活血方对胎儿生长受限(fetal growth restriction,FGR)孕鼠胎盘滋养细胞凋亡的影响,探讨该方治疗FGR的机制。方法采用被动吸烟法建立大鼠FGR模型,将32只孕鼠随机分为正常组、模型组、中药组(采用补肾益气活血方治疗)、西药组(采用精氨酸治疗)。运用HE染色观察各组胎盘的组织学变化,TUNNEL法检测胎盘滋养细胞的凋亡及RT-PCR、免疫组化检测胎盘滋养细胞Bcl-2、Bax的表达。结果模型组胎盘绒毛出现血液淤积及缺血,中药可明显改善FGR胎盘绒毛的微循环,而西药组则部分改善。正常组、模型组、中药组、西药组的绒毛两层合体细胞凋亡指数中位数依次为45%、75%、57%、70%,正常组和中药组的凋亡指数均低于模型组(P<0.01),西药组与模型组比较差异无统计学意义。各组胎盘Bcl-2、Bax的mRNA和蛋白表达比较,差异均无统计学意义。结论补肾益气活血方可改善FGR胎盘微循环,防止FGR胎盘滋养细胞的过度凋亡,但其并非通过经典的Bax/Bcl-2凋亡途径起作用,其作用途径仍待探讨。Objective To observe the effect of Bushen Yiqi Huoxue Recipe （BYHR,a Chinese medical prescription for reinforcing Shen,replenishing qi and promoting blood circulation） on placental trophoblast apoptosis in fetal growth restricted （FGR） pregnant rat for the sake of explore its mechanism of action in treating FGR.Methods FGR animal model was established by passive smoking,32 pregnant rats were divided into four groups at random：the normal group,the model group,the Chinese medicine （CM） group （model rats treated by BYHR） and the Western medicine （WM） group （model rats treated by arginine）. The histological change of placenta was examined with HE stain,the trophoblast apoptosis was detected by TUNEL and RT-PCR,and the expressions of Bcl-2 and Bax in the placenta were detected by immunohistochemical method. Results Blood stasis and villous ischemia were seen in placenta of FGR model rats. Placental microcirculation was significantly improved in the CM group,but in the WM group only partially improved. The median apoptotic index of syncytial trophoblast cells in the four groups,in normal,model,CM,and WM order,was 45%,75%,57% and 70%,as compared with the model group,it was much lower in the normal group and the CM group （P0.01）,but a similar level was shown in the WM group. No significant difference in mRNA and protein expressions of Bcl-2 and Bax was found among the four groups. Conclusion BYHR can improve the placental microcirculation in FGR rats to prevent excessive apoptosis through a mechanism other than the classical Bax/Bcl-2 apoptotic pathway,which needs further exploring.

关 键 词：胎儿生长受限 补肾益气活血方 胎盘 凋亡 

分 类 号：R285.5[医药卫生—中药学]