X射线诱发大鼠淋巴细胞TCR基因突变的剂量-效应关系  

The Dose-response Relationship of T-cell Receptor Gene Mutation of Rat Peripheral Blood Lymphocytes Induced by X-ray.

作　　者：石燕[1] 刘伟[1] 侯殿俊[1] 马娅[1] 乔建维[1] 卢峰[1] 商希梅[1] 

机构地区：[1]山东省医学科学院放射医学研究所,山东济南250062

出　　处：《中国辐射卫生》2010年第2期138-139,共2页Chinese Journal of Radiological Health

基　　金：山东省自然科学基金(Y2007C151)

摘　　要：目的探讨X射线诱发大鼠外周血淋巴细胞TCR基因突变的剂量-效应关系。方法以不同剂量（0~3Gy）的X射线照射新鲜分离的大鼠外周血淋巴细胞,PHA、ConA、IL-2协同刺激培养7d,流式细胞仪检测TCR突变频率。结果大鼠淋巴细胞体外培养7d后,TCR突变频率随照射剂量的增加而增加,二者存在良好的剂量-效应关系,最佳拟合曲线为二次多项式模式,回归方程为：TCRMF=1.615＋9.979D＋1.712D2（F=146.781,P〈0.01,R2adj=0.864）。结论本研究结果显示：X射线诱发大鼠外周血淋巴细胞TCR基因突变频率与照射剂量间存在良好的剂量-效应关系。Objective To investigate the does-response relationship of T-cell receptor from rat peripheral blood lymphocyte induced by X-ray.Methods Freshly isolated peripheral lymphocytes from rat were irradiated with X-ray ranged from 0 Gy to 3 Gy and cultured with phytohemagglutinin Concanavalin A and interleukin-27days,The mutant frequencies of TCR gene were detected by flow cytometry.Results TCR genes mutation increases with the dose incense in the cultured the rat lymphocytes 7 days and it exists favourable dose-response relationship.Data were found to be fitted out optimal by a quadratic polynomial dose-response modle,which could be described by regression equation： TCRMF= TCRMF=1.615＋9.979D＋1.712D2（F=146.781,P0.01,R2adj=0.867）.Conclusion The favourable dose-response relationship between TCRMF induced by X ray and dose can be found from this study.

关 键 词：TCR 剂量-效应关系 生物剂量计 

分 类 号：Q691[生物学—生物物理学]