Leptin对THP-1细胞增殖和吞噬功能的影响及机制的初步研究  

Effect of Leptin on Activity of Proliferation and Phagocytosis in THP-1 Cells and Its Mechanism

作　　者：阳大庆[1] 石丽萍[1] 梁进军[2] 王林[1] 周细国[3] 

机构地区：[1]湖南怀化医学高等专科学校,湖南怀化418000 [2]湖南省疾病预防控制中心 [3]怀化市第一人民医院

出　　处：《实用预防医学》2010年第6期1049-1051,共3页Practical Preventive Medicine

基　　金：湖南省教育厅优秀青年基金(编号07B059)

摘　　要：目的探讨瘦素对人单核细胞系THP-1增殖和吞噬活性的影响,为阐明瘦素在抗感染免疫中的作用提供依据。方法采用CCK-8法检测瘦素处理前后THP-1细胞的增殖活性,采用流式细胞术测定瘦素处理前后THP-1细胞对白色念珠菌的吞噬情况,RT-PCR检测甘露糖受体(MR)的表达情况。结果经瘦素处理后,THP-1细胞出现明显增殖(P<0.01),且呈现明显的剂量-效应关系。瘦素处理后的THP-1细胞对白色念珠菌的吞噬也明显增强,但RT-PCR结果显示这种增强与MR表达之间无必然联系。结论瘦素可以明显刺激THP-1细胞增殖和吞噬活性的增强。Objective To investigate the effect of leptin on the activity of proliferation and phagocytosis in THP-1 cells,and to provide the evidence for elucidating the role of leptin in immune regulation.Methods Before and after leptin treatment,the activity of proliferation in THP-1 cells was detected by CCK-8 spectro-photometry.Flow cytometry was used to measure the phagocytosis of THP-1 cells.The expression of MR was determined by RT-PCR.Results After leptin treatment,the activity of proliferation in THP-1 cells was increased significantly （P〈0.01） and showed a very clear dose-response relation.The activity of phagocytosis was also increased significantly after leptin treatment,but the increase was not regulated by mannose receptor.Conclusions By up-regulating the activity of proliferation and phagocytosis in THP-1 cells,leptin might affect the functions of THP-1 cells.

关 键 词：瘦素 THP-1 增殖 吞噬功能 甘露糖受体 

分 类 号：R392.12[医药卫生—免疫学]