血管紧张素Ⅱ受体在肾上腺产醛固酮腺瘤中的表达研究  

Expression of Angiotensin Ⅱ Receptor in Aldosterone-producing-adenoma

作　　者：倪栋[1] 吴准[1] 王保军[1] 张国玺[1] 闫永吉[1] 龚道静[1] 李俊[1] 欧阳金枝[2] 张旭[1,3] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030 [2]解放军总医院内分泌科 [3]解放军总医院泌尿外科

出　　处：《临床泌尿外科杂志》2010年第5期367-370,共4页Journal of Clinical Urology

基　　金：国家杰出青年科学基金资助项目(编号30725040)

摘　　要：目的:研究血管紧张素Ⅱ受体AT_1R和AT_2R在肾上腺产醛固酮腺瘤(APA)中的表达。方法:通过逆转录聚合酶链反应(RT-PCR)检测50例APA及瘤旁组织和12例正常肾上腺组织中AT_1R和AT_2R mRNA表达,采用免疫组织化学染色方法检测组织石蜡切片中AT_1R和AT_2R蛋白表达情况。结果:AT_1R在腺瘤、瘤旁及正常肾上腺组织中的表达无明显差异。AT_2R在APA组织中的表达低于正常肾上腺组织(P<0.05)。将AT_2R mRNA表达量与患者临床数据作相关性分析,提示AT_2R的表达与患者血浆醛固酮浓度负相关(r=-0.467,P<0.05),与血浆肾素活性正相关(r=0.604,P<0.05)。结论:AT_2R表达下调可能与肾上腺产醛固酮腺瘤原发性醛固酮增多症的发病相关。Objective： To investigate the expression of angiotensin Ⅱ receptors AT1R and AT2R in aldoste rone-producing-adenoma （APA）. Methods：RT PCR was used to detect the AT1R and AT2R mRNA isolated from the adenomas and non-adenoma portions of 50 APAs and 12 normal adrenal tissues. Immunohistochemistry was used to detect the expression of AT1 and AT, receptors in the APA and normal adrenal cortical tissue. Results： There was no significant difference between APA and normal adrenal cortical tissue in AT1 receptor expression. The expression of AT2 receptor in APA was less than that in normal adrenal cortical tissue （P〈0.05）. The analysis of the correlation between the AT2R mRNA expression and the clinical data showed that the AT2R mRNA expression was correlated negatively with the plasma aldosterone concentration（r= -0. 467, P〈0.05） and posi tively with the plasma renin activity （ r = 0. 604, P〈 0.05 ）. Conclusions： The down-regulated expression of AT2 R may be associated with the etiological factor of APA.

关 键 词：原发性醛固酮增多症 肾上腺皮质腺瘤 血管紧张素Ⅱ1型受体 血管紧张素Ⅱ2型受体 

分 类 号：R586.2[医药卫生—内分泌]