直线加速器小剂量X线照射对BALB/c小鼠T细胞亚群影响的动态观察研究  

Dynamic observation to T cell subsets of BALB/c mice after irradiation with low-dose X-ray

作　　者：王存邦[1] 白海[1] 葸瑞[1] 薛志文[1] 

机构地区：[1]兰州军区兰州总医院全军血液病中心,甘肃兰州730050

出　　处：《西北国防医学杂志》2010年第3期170-172,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：全军"十一五"杰出人才基金资助项目(编号06J005);兰州军区医药卫生科研基金资助项目(LXH-2007006)

摘　　要：目的：观察小剂量X线照射对BALB/c小鼠T细胞亚群的影响。方法：小鼠按实验分组经直线加速器2和3 Gy X线照射,流式细胞仪动态测定T细胞亚群变化。结果：照射后,小鼠外周血CD3＋、CD4＋、CD8＋细胞呈进行性降低,至第3、4周出现逐渐恢复迹象,4、5周后恢复正常。在同一时间,3 Gy较2 Gy照射所导致的细胞亚群降低的程度更为明显（P〈0.05）。结论：BALB/c小鼠经照射后T细胞亚群下降明显,4~5周后恢复正常。Objective：To explore the effect of irradiation with low-dose X-ray on T cell subsets of BALB/c mice.Methods：Mice were divided into three groups according to experimental design,and were irradiated with 2 Gy or 3 Gy with linear accelerator respectively.T cell subsets were detected by flow cytometry.Results：In merely irradiation group,the CD3＋,CD4＋,CD8＋ cells in peripheral blood decreased progressively,began to recover gradually after 3 to 4 weeks,and recovered to normal level after 4 to 5 weeks.At the same time points,the reduction of T cell subsets in 3-Gy irradiation group was more obvious than that in 2-Gy irradiation group（P0.05）.Conclusion：T cell subsets of BALB/c mice may reduce obviously after irradiation with low-dose X-ray and recover gradually after 4-5 weeks.

关 键 词：直线加速器 小剂量X线 照射 BALB/C小鼠 T细胞亚群 

分 类 号：R392[医药卫生—免疫学]