ACE2在小鼠胰腺组织中的表达  

Expression of ACE2 in Mouse Pancreas Tissue

作　　者：郭丽敏[1] 王夏涟 林珊珊[1] 杨金奎[2] 

机构地区：[1]北京市石景山医院内分泌科 [2]首都医科大学附属北京同仁医院内分泌科

出　　处：《首都医科大学学报》2010年第3期359-362,共4页Journal of Capital Medical University

基　　金：国家自然科学基金(30671001)资助项目~~

摘　　要：目的检测SARS冠状病毒(SARS-CoV)S蛋白的功能性受体血管紧张素转换酶2(ACE2)在小鼠胰腺内、外分泌腺的表达。方法①RT-PCR:麻醉小鼠后取胰腺和肺组织,采用RT-PCR方法检测各组织ACE2的表达。②免疫电镜:麻醉小鼠后取胰尾,采用免疫电镜方法检测胰腺组织ACE2的表达。结果胰腺和肺脏组织均表达ACE2mRNA,且胰腺内、外分泌腺均表达ACE2。结论 ACE2在小鼠胰腺内、外分泌腺的表达可介导SARS-CoV侵入并损害胰腺,使胰岛素耗竭。Objective This study was designed to investigate the expression of angiotensin-converting enzyme 2（ACE2） in endocrine and exocrine tissue of mouse pancreas. Methods The expressions of ACE2 in mouse pancreas was detected by reverse transcriptase polymerase chain reaction（RT-PCR） and the expressions of ACE2 in mouse pancreas was detected by immuno-electron microscopy. Results The expression of ACE2 mRNA was detected in whole mouse pancreas and lung tissues,while ACE2 protein was detected in both endocrine tissue and exocrine tissue of mouse pancreas. Conclusion These data demonstrated a tissue specific pattern of ACE2 expression in mouse pancreas and this indicated ACE2 might be present in islet of pancreas of human. Since ACE2 works as a negative regulator of the angiotensin system,the expression of ACE2 protein in endocrine tissue of pancreas may give us more clue for the treatment of diabetes.

关 键 词：血管紧张素转换酶2 SARS冠状病毒 S蛋白 胰腺 

分 类 号：R972.4[医药卫生—药品] R977.3[医药卫生—药学]