运动训练对血管性痴呆大鼠学习记忆能力及大脑皮质区生长相关蛋白-43表达的影响  

The effects of exercises training on learning,memory and expression of GAP-43 in cortex area of rat with vascular dementia

作　　者：樊振勇[1] 陈丽娜[1] 徐琳峰[1] 纵亚[1] 胡坚勇[1] 于向华[1] 顾伟忠[2] 

机构地区：[1]杭州市第一人民医院康复科,杭州310006 [2]浙江大学附属儿童医院病理科

出　　处：《中国康复医学杂志》2010年第6期514-518,共5页Chinese Journal of Rehabilitation Medicine

基　　金：杭州市医药卫生科技计划项目资金资助(2006B001)

摘　　要：目的:研究运动训练对血管性痴呆大鼠学习记忆功能恢复及组织生长相关蛋白(GAP-43)表达的影响。方法:采用双侧颈总动脉反复缺血再灌注加降血压法制作血管性痴呆大鼠模型。选择SD雌性大鼠44只,随机分为运动组20只,制动组20只和假手术组4只。运动组大鼠进行滚筒、转棒训练,1h/d;制动组大鼠限制其自由活动;假手术组置于普通笼中自由活动。3组分别于术后第27、28天进行跳台实验测定学习、记忆能力。取脑组织采用免疫组织化学染色方法观察不同时间点大脑皮质区GAP-43的表达。结果:跳台实验对学习记忆能力进行评估,运动组优于制动组(P<0.01),运动组GAP-43在皮质区的表达第1天逐渐增高,第7天达高峰,较制动组和假手术组均有明显增加(P<0.01)。结论:运动训练可改善VD大鼠学习记忆能力,其机制可能与运动训练能促进皮质区上GAP-43表达有关。Objective：To study the effects of exercises training on the improvement of learning and memory ability and the expression of growth associated protein-43（GAP-43） in vascular dementia rats.Method：The imitative vascular dementia model was established by repeatedly clipping the common carotid arteries of both side for ischemia-reperfusion and reducing blood pressure with an abdominal injection of sodium nitroprusside.Forty-four female SD rats were randomly assigned to exercises training group（20 cases）,immobilization group（20 cases）,and sham-operation group（4 cases）.The rats in exercises training group were administered exercises with a rotating bar and rolling tube for 1h daily,while those in immobilization group were immobilized without any exercises;the rats in the sham-operation group can free exercises in cage.Learning and memory tests were preformed by using step-down avoidance test at the 27th d,28th d after operation.Immunohistochemical staining was used to detect the GAP-43 expression around the hippocampus CA1 area at different time points after operation.Result：Exercises training group showed better learning and memory ability and more GAP-43 expressions in the cortex area than immobilization group（P0.01） and sham-operation group（P0.01）.Conclusion：Exercises training can improve ability of learning and memory in vascular dementia rats,and the mechanism possibly is related to the increase of GAP-43 in the cortex area.

关 键 词：血管性痴呆 运动训练 学习记忆 生长相关蛋白-43 

分 类 号：R87[医药卫生—运动医学] R749.16[医药卫生—临床医学]