幼年大鼠神经根断裂后移植修复对神经元凋亡基因表达影响的研究  

The impact of graft repair of ruptured nerve roots in young rats on the expression of neuron apoptotic genes： an experimental study

作　　者：傅阳[1] 陈亮[1] 顾玉东[1] 

机构地区：[1]复旦大学附属华山医院手外科,上海200040

出　　处：《中华手外科杂志》2010年第3期130-133,共4页Chinese Journal of Hand Surgery

基　　金：基金项目：国家973计划课题基金资助项目（2003CB515305）

摘　　要：目的观察幼年大鼠神经根损伤后即行神经移植修复对近端神经元C—Jun和Bcl-2表达水平的影响。方法将出生18dSD大鼠24只等分为2组：神经根切断组，将右侧颈5神经根切除0．3cm；神经根修复组，颈，神经根切除后取腓肠神经移植修复。于术后4周取颈5脊髓和背根神经节。通过RT-PCR检测大鼠近端神经元C—Jun和Bcl-2mRNA的表达水平，并比较两组间的差异。结果与神经根切断组相比，神经根修复组神经元C—Jun mRNA的表达水平明显下降，Bcl-2mRNA的表达水平显著升高。结论幼年大鼠凋亡基因表达水平变化是产瘫早期臂丛神经修复手术保护近端神经元的分子机制。Objective To investigate the impact of immediate graft repair of transected nerve root on expression of C-Jun and Bcl-2 mRNA in proximal neurons in young rats. Methods Twenty-four 18-day-old SD rats were divided into two groups. In the nerve root resection group a 0.3 cm segment of C5 nerve root was resected. In the graft repair group resection of a 0.3 em segment of C5 nerve mot was followed by suml nerve grafting. C5 level spinal cord and dorsal root ganglion were harvested 4 weeks postoperatively. Real time reverse transcription polymerase chain reaction （RT-PCR） was carried out to measure the expression of C-Jun and Bcl-2 mRNA in the proximal neurons. Differences between the two groups were compared. Results Compared with C5 resection group, C-Jun mRNA in the proximal motor and sensory neurons of C5 graft repair group was significantly lower while Bcl-2 mRNA was sigrfifieanfly higher. Conclusion The difference in apoptotie gene expression in proximal neurons is likely the molecular mechanism for the neuronal protective effect of nerve grafting in brachial plexus birth palsy.

关 键 词：臂丛 神经元 细胞凋亡 实时定量RT-PCR 

分 类 号：R285.5[医药卫生—中药学]