当归多糖预防给药对放射损伤小鼠骨髓单个核细胞黏附分子表达及细胞周期的影响  

Effect of Prophylactic Administration with Angelica Polysaccharides on Expression of Adhesion Molecules and Cell Cycle of Bone Marrow Mononuclear Cells of Radiation Injured Mice

作　　者：张雁[1] 吴宏[1] 关雪晶[1] 周玥[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室基础医学院组胚教研室,重庆400016

出　　处：《中国生物制品学杂志》2010年第6期627-632,共6页Chinese Journal of Biologicals

基　　金：重庆市自然科学基金计划项目(2007BB5284)

摘　　要：目的探讨当归多糖(Angelica polysaccharide,APS)预防给药对放射损伤小鼠骨髓单个核细胞(Bone marrow mononuclear cells,BMNCs)黏附分子表达及细胞周期的影响。方法将BALB/c小鼠分为4组:正常对照组(不作任何处理)、NS组(经腹腔注射生理盐水)、2mg/kgAPS组(经腹腔注射2mg/kgAPS)和8mg/kgAPS组(经腹腔注射8mg/kgAPS),连续注射7d后,进行照射,并继续给药13d。分别于照射后第7、14天采集各组小鼠外周血,并取骨髓,进行WBC、RBC、PLT及BMNC计数;流式细胞术检测小鼠Sca-1+BMNC表面黏附分子CD44和CD49d的表达及BMNC细胞周期的变化;半定量RT-PCR和Western blot法分别检测小鼠BMNC细胞周期蛋白D(2CyclinD2)mRNA转录水平和蛋白表达水平的变化。结果与正常对照组比较,NS组外周血WBC、RBC、PLT及BMNC数量均明显减少,Sca-1+BMNC表面黏附分子CD44和CD49d的表达明显下降(P<0.05),G0/G1期细胞比例显著增加(P<0.05),CyclinD2 mRNA转录水平和蛋白表达水平均明显降低(P<0.05);2mg/kgAPS组和8mg/kgAPS组能增加外周血各指标及BMNC数量(P<0.05),明显降低第7和14天Sca-1+BMNC表面黏附分子CD44和CD49d的表达水平(P<0.05),降低G0/G1期细胞比例(P<0.05),提高CyclinD2 mRNA的转录水平和蛋白表达水平(P<0.05)。结论 APS预防给药能通过降低放射损伤小鼠Sca-1+BMNC表面黏附分子的表达水平,上调BMNC的CyclinD2 mRNA和蛋白表达来加速BMNCG1期向S期的转换,促进造血恢复。Objective To investigate the effect of prophylactic administration with Angelica polysaccharides（APS）on the expression of adhesion molecules and cell cycle of bone marrow mononuclear cells（BMNCs）of radiation injured mice.Methods BALB /c mice were divided into one normal control,one normal saline（NS）control and two test groups.The mice in normal control group were untreated,while those in NS control and the two test groups were injected i.p.with NS as well as 2 and 8 mg /kg APS respectively,once a day for 7 d,then subjected to radiation and further injected for 13 d.The peripheral blood of mice in various groups were collected on days 7 and 14 after radiation and counted for WBC,RBC and PLT,while the bone marrow were collected and counted for BMNCs.The expressions of adhesion molecules CD44 and CD49d on surface as well as cell cycle of Sca-1＋BMNCs were determined by flow cytometry.The transcription level of CyclinD2 mRNA in BMNCs was determined by semi-quantitative RTPCR,while the expression level of CyclinD2 by Western blot.Results Compared with those in normal control group,the counts of WBC,RBC,PLT and BMNCs,the expression levels of CD44 and CD49d on surface of Sca-1＋BMNCs,as well as CyclinD2 mRNA transcription and protein expression levels of mice in NS control group decreased significantly（P〈0.05）,while the percentage of cells at G0 /G1 phase increased significantly（P〈0.05）.However,the counts of WBC,RBC,PLT and BMNCs as well as CyclinD2 mRNA transcription and protein expression levels of mice in the two test groups increased significantly（P〈0.05）,while the expression levels of CD44 and CD49d on surface of Sca-1＋BMNCs and the percentage of cells at G0 /G1 phase decreased significantly（P〈0.05）.Conclusion Prophylactic administration with APS accelerated the cell cycle of BMNCs from G1 phase to S phase and promoted the recovery of hematopoietic function by decreasing the expression levels of adhesion molecule on surface of Sca-1^＋ BMNCs and up-regulating the expressions

关 键 词：当归多糖 骨髓 单核细胞 抗原 CD44 CD49D 细胞周期 细胞周期蛋白D2 

分 类 号：R285[医药卫生—中药学]