重组人睫状神经营养因子生物学活性检测方法的建立  

Development of A Method for Determination of Biologic Activity of Recombinant Human Ciliary Neurotrophic Factor

作　　者：李萍[1] 蒋琳[1] 马亚茹[1] 应莲芳[1] 卜晓萍[1] 

机构地区：[1]兰州生物制品研究所第四研究室,兰州730046

出　　处：《中国生物制品学杂志》2010年第6期646-648,653,共4页Chinese Journal of Biologicals

摘　　要：目的建立重组人睫状神经营养因子(Recombinant human ciliary neurotrophic factor,rhCNTF)的生物学活性检测方法 ,在其研制和生产中进行有效的质量控制。方法应用鸡胚睫状神经节细胞培养法和鸡胚背根神经节法,对rhCNTF进行定性和半定量检测;应用TF-1.CN5a.1细胞增殖法检测rhCNTF的活性;应用正常小鼠减重法,对rhCNTF的减肥生物学活性进行检测。并探索4种方法的实验条件及优缺点。结果上述4种方法均能用于rhCNTF的生物学活性测定,以TF-1.CN5a.1细胞增殖法及正常小鼠减重法结合使用最为有效。结论可联合使用TF-1.CN5a.1细胞增殖法和正常小鼠减重法,对重组rhCNTF进行质量控制。Objective To develop a method for determination of biologic activity and for effective quality control during preparation and production of recombinant human ciliary neurotrophic factor（rhCNTF）.Methods Qualitative and semi-quantitative determinations of rhCNTF were carried out by cultures of ciliary ganalia cells and dorsal root ganglia of chicken embryo respectively.The activity of rhCNTF was determined by TF-1.CN5a.1 cell proliferation method.The biologic activity of rhCNTF in obesity was determined by bodyweight decrease test in normal mice.The four methods were investigated for experimental conditions and evaluated for advantages and shortcomings.Results All the four methods were suitable for the determination of biologic activity of rhCNTF.However,the TF-1.CN5a.1 cell proliferation method combined with bodyweight decrease test in normal mice was more effective.Conclusion The quality of rhCNTF may be controlled by TF-1.CN5a.1 cell proliferation method combined with bodyweight decrease test in normal mice.

关 键 词：睫状神经营养因子 神经节 脊 生物学活性 

分 类 号：Q819[生物学—生物工程]