二乙烯亚胺对脊髓灰质炎病毒Ⅱ型Sabin株的灭活效果  

Inactivation Effect of Binary Ethylenimine on Type Ⅱ Poliovirus Sabin Strain

作　　者：王白燕[1] 张新文[1] 蔡玮[1] 马磊[1] 高菁霞[1] 孙明波[1] 姜述德[1] 李卫东[1] 杨静思[1] 廖国阳[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所生物制品五室,昆明650118

出　　处：《中国生物制品学杂志》2010年第6期649-653,共5页Chinese Journal of Biologicals

基　　金：云南省重点新产品开发项目(2008CA035);国家重大新药创制(2008ZX09101-031);云南省技术创新人才项目(2003PY07);国家支撑计划(2008BAI66B04)

摘　　要：目的观察二乙烯亚胺(Binary ethylenimine,BEI)对脊髓灰质炎病毒Ⅱ型Sabin株的灭活效果。方法在不同温度(37、33、28和25℃)下采用4种浓度(0.002、0.001、0.0005和0.00025mol/L)的BEI及0.0925mg/ml的甲醛对脊髓灰质炎病毒Ⅱ型Sabin株进行灭活,采用微量滴定法测定病毒感染性滴度,ELISA法检测灭活病毒液D抗原含量,并进行病毒灭活验证试验。结果在37℃条件下,0.002mol/L的BEI能在48h内完全灭活病毒,灭活后的病毒液D抗原含量回收率为90.6%,显著高于甲醛灭活的回收率(60.6%)。经验证,灭活彻底,无活病毒存在。结论 BEI对脊髓灰质炎病毒Ⅱ型Sabin株的D抗原破坏小,能较好地保持疫苗的有效成分。Objective To observe the inactivation effect of binary ethylenimine（BEI）on type Ⅱ poliovirus Sabin strain.Methods Sabin strain was inactivated at various temperatures（37,33,28 and 25℃）with BEI at various concentrations（0.002,0.001,0.0005 and 0.00025 mol /L）,and determined for infectious titer of virus by microtitration and for D antigen contents by ELISA,using those inactivated with formaldehyde as control,based on which the verification test on virus activation was performed.Results Sabin strain was completely inactivated with 0.002 mol /L BEI at 37℃ for 48 h.The recovery rate of D antigen content of the inactivated virus was 90.6%,which was significantly higher than that inactivated with formaldehyde（60.6%）.No live virus was detected by verification test.Conclusion BEI showed less damage to D antigen of type Ⅱ poliovirus Sabin strain and good protection to active constituents of vaccine.

关 键 词：脊髓灰质炎病毒 二乙烯亚胺 病毒灭活 

分 类 号：R373.2[医药卫生—病原生物学] R392.33[医药卫生—基础医学]