兔VX2肝癌模型应用索拉非尼前后的CT灌注成像与组织学对照研究  

Control study of CT perfusion imaging and histology of rabbit liver VX2 tumor before and after the application of Sorafenib

作　　者：吴越[1] 梁长虹[1] 杨本强[2] 刘文源[2] 李子浩[2] 

机构地区：[1]广东省人民医院,广东省医学科学院放射科,广东广州510080 [2]沈阳军区总医院放射诊断科,辽宁沈阳110016

出　　处：《中国临床医学影像杂志》2010年第6期401-404,408,共5页Journal of China Clinic Medical Imaging

摘　　要：目的:通过对兔VX2肝癌模型应用索拉非尼前后的CT灌注参数与组织学进行研究,探索应用CT灌注成像这种影像学手段来监测肿瘤生长的可行性,同时为评价索拉非尼抑制肿瘤血管生成和促进肿瘤细胞凋亡的效果提供实验依据。方法:12只新西兰大白兔,采用开腹瘤块包埋法建立兔VX2肝癌模型,2只建模后处死作为病理对照,其余10只于种瘤后第14d行多层螺旋CT灌注扫描,对比肿瘤区与非肿瘤区肝脏的肝血流量(HBF)、肝血容积(HBV)、平均通过时间(MTT)、毛细血管表面通透性(PS)和肝动脉分数(HAF)的参数值。然后经口给予索拉非尼(20mg/kg/d)7d,种瘤后第21d再次行CT检查,两次扫描肿瘤区所获得的参数值进行比较。检查完成后处死实验兔,进行病理与细胞凋亡(Caspase-3)免疫组织化学染色。结果:12只兔均接种成功。种瘤后第14d肿瘤区HBF、HBV、PS、HAF增高,与非肿瘤区肝组织比较有统计学差异(P<0.01)。肿瘤区MTT降低,与非肿瘤区肝组织比较有统计学差异(P<0.01)。两次扫描肿瘤区所获得的参数HBF、PS、HAF值比较差异有显著性意义(P<0.01)HBV、MTT值差异无显著性意义。Caspase-3表达强度明显低于对照组(P<0.05)。结论:多层螺旋CT灌注成像能较好地评价兔VX2肝癌模型的血流动力学变化,结合了组织学的研究则可以更加明确索拉非尼抑制肿瘤血管生成和促进肿瘤细胞凋亡的作用。Objective： To study the CT perfusion parameter change and histology of rabbit liver VX2 tumor before and after the application of Sorafenib, it was supposed to explore the feasibility of CT perfusion imaging in monitoring of the growth of liver tumor, and to provide experimental basis for evaluation the effect of Sorafenib inhibiting tumor angiogenesis and promoting cell apoptosis. Methods： The multi-slice CT perfusion was performed in 12 New Zealand white rabbits at 2 weeks after VX2 hepatic tumor implantation. Two were executed as the control group of pathology. The various perfusion parameters （HBF, HBV, MTT, PS, HAF） were measured in the tumor area and non-tumorous regions in other 10 rabbits. Sorafenib was administered by mouth at the dosage of 20mg/kg/d for one week. The second CT perfusion was performed at 3 weeks, the parameters of tumor region were compared by the previous result. After that, all the rabbits were executed and pathological examination and Caspase-3 staining was performed. Result： Twelve （100%） rabbits were successfully implanted with VX2 tumor in the liver. It was demonstrated that the increased HBF, HBV, PS, HAF and the decreased MTT in the tumor area were dif- fered significantly from the non-tumorous regions at 2 weeks after VX2 hepatic tumor implantation （P〈0.01）. The statistical analysis also showed significant difference in HBF, PS, HAF of liver tumor at the two CT perfusion （P〈0.01）. And no significant difference in HBV, MTT. The expression intensity of Caspase-3 was significantly lower than that of normal control group （P〈0.05）. Conclusion： The multi-slice CT perfusion imaging had advantages in the changes of hemodynamics of the rabbit model of VX2 liver tumor, the effect of Sorafenib inhibiting tumor angiogenesis and promoting cell apoptosis could be more identified if combination with the research of histology.

关 键 词：肝肿瘤 实验性 体层摄影术 X线计算机 

分 类 号：R735.7[医药卫生—肿瘤] R814.42[医药卫生—临床医学]