河北唐山汉族人群VDR和MBP基因交互作用与肺结核发病的相关性  

Relationship of gene-gene interaction of VDR and MBP loci with susceptibility to pulmonary tuberculosis of Han population in Tangshan Hebei

作　　者：冯福民[1] 郝金奇[1] 陈怡[1] 孙淑丰[2] 郑国颖[1] 陈刚[1] 

机构地区：[1]华北煤炭医学院流行病与卫生统计学科,河北省煤矿卫生与安全重点实验室,河北唐山063000 [2]华北煤炭医学院护理学系中心实验室,河北唐山063000

出　　处：《重庆医学》2010年第12期1527-1529,1532,共4页Chongqing medicine

基　　金：河北省教育厅博士基金项目(B2004127)

摘　　要：目的探讨维生素D受体(VDR)和甘露糖结合蛋白(MBP)基因多态性及其交互作用与汉族成年人肺结核发病的关系。方法选择汉族成年人新发肺结核患者182例和成年健康体检者190例组成病例组和对照组。调查研究对象的一般情况,分别以PCR-RFLP、特异引物PCR方法分析VDR和MBP基因多态性。结果病例组和对照组VDR基因Taq Ⅰ、Apa Ⅰ、Fok Ⅰ及MBP基因P52、P54位点突变型等位基因频率分别为4.0%、29.1%、49.2%、8.6%、6.7%和6.1%、28.2%、46.1%、3.9%、6.3%。携带P52突变型基因为肺结核发生的危险因素。FokⅠFf与ApaI Aa、Fok Ⅰ Ff与P52突变基因型之间存在交互作用,OR及其95% CI分别为2.170(1.198～3.931)和6.063(1.324～27.756)。同时携带Fok Ⅰ Ff与ApaI Aa者发生肺结核的危险性提高2.5倍。结论 VDR基因Fok Ⅰ杂合子与ApaI杂合子或MBP基因P52突变型之间均存在协同作用。Objective To study the relationship of the genetic polymorphisms of Vitamin D receptor（VDR） and Mannose binding protein （MBP） and susceptibility to pulmonary tuberculosis and the gene-gene interaction in a Chinese Population in Tangshan area.Methods One hundred and eighty-two cases and 190 controls were collected and a case-control study was conducted.Information on related risk factors of tuberculosis was collected.Polymerase chain reaction with restriction fragment length polymorphism（PCR-RFLP）and sequence-specific primers（PCR-SSP）were carried out in the polymorphism in VDR and MBP gene, respectively.The Fok Ⅰ, Taq Ⅰ and Apa Ⅰ enzyme were employed.Results The frequency of mutant allele of VDR and MBP gene on Fok Ⅰ, Taq Ⅰ, Apa Ⅰ,P52 and P54 were 4.0%,29.1%,49.2%,8.6%,6.7% and 6.1%,28.2%,46.1%,3.9%,6.3% in cases and controls, respectively.The univariate and multivariate logistic analyses demonstrated that P52 gene mutant were risk factors to pulmonary tuberculosis.The interaction was confirmed on Fok Ⅰ Ff and ApaI Aa,Fok Ⅰ Ff and P52 mutant with OR（95%CI）being 2.170（1.198-3.931） and 6.063（1.324-27.756） respectively.Who held Fok Ⅰ Ff and ApaI Aa will be likely to induce pulmonary tuberculosis, the risk increased 2.5 times.Conclusion The synergetic effect is proved on susceptibility to pulmonary tuberculosis between Fok Ⅰ Ff and ApaI Aa,Fok Ⅰ Ff and P52 mutant genetype.

关 键 词：肺结核 维生素D受体 甘露糖结合蛋白 基因多态性 交互作用 

分 类 号：R521.02[医药卫生—内科学]