丹参酮对脊髓缺血再灌注损伤大鼠模型脊髓、血清谷氨酸含量的影响  

Influence of tanshinone on glutamic acid content in spinal cord and serum in a rat model of spinal cord ischemia/reperfusion injury

作　　者：林翔[1] 张俐[2] 刘蔚楠[2] 聂光瑞[2] 伏勇[2] 曾启清[2] 

机构地区：[1]福建中医学院附属人民医院,福州350001 [2]福建中医学院骨伤系,福州350001

出　　处：《海峡药学》2010年第5期49-51,共3页Strait Pharmaceutical Journal

基　　金：福建省自然基金项目(C0510023)

摘　　要：目的观察丹参酮对脊髓缺血再灌注大鼠脊髓、血清谷氨酸水平探讨丹参酮保护脊髓的作用机制。方法选用SD大鼠88只,采用结扎腹主动脉的方法制作脊髓缺血再灌注模型。按随机数字表法将动物分为假手术组(n=8)、模型组(n=40)和丹参酮组(n=40)。A组在全麻下充分暴露脊髓,B组及C组采用Zivin法改进方法复制模型,随机在脊髓缺血再灌注30min、1h、4h、8h、12h的相应时间点切取脊髓,抽取下腔静脉血,检测缺血再灌注后脊髓谷氨酸、血清谷氨酸含量及对缺血再灌注4h、8h、12h,采用改良Tarlov评分标准进行神经功能评分。结果丹参酮组及模型组大鼠脊髓、血清谷氨酸含量均于脊髓缺血再灌注损伤后30min开始上升,缺血再灌注损伤4h后含量达到高峰,其后含量逐渐下降,12h后基本恢复正常。丹参酮组各观测点脊髓、血清谷氨酸含量均低于模型组。结论丹参酮能降低脊髓缺血再灌注大鼠脊髓谷氨酸含量,对大鼠脊髓缺血再灌注损伤具有保护作用。OBJECTIVE To explore the interventional effect of tanshinone on glutamic acid content in spinal cord and in serum and neurological function in a rat model of spinal cord ischemia/reperfusion injury.METHODS A total of 88 Sprague Dawley rats were randomly divided into a sham operation（n=8）,model（n=40）,and tanshinone（n=40） groups.Abdominal aorta occlusion was performed along the right renal arterial root using a Scoville-Lewis clamp to induce spinal cord ischemia.Blood flow was recovered 30 minutes following occlusion to establish models of spinal cord ischemia/reperfusion injury.Abdominal aorta occlusion was not performed in the sham operation group.An intraperitoneal injection of tanshinone IIA sulfonic sodium solution was administered to rats in the tanshinone group,preoperatively.In addition,rats in the sham operation and model groups were treated with an intraperitoneal injection of the same concentration of saline,preoperatively.Tirty minutes after ischemia,rats in the model and tanshinone groups were observed at 30min,hour1,4,8,and 12 following perfusion,with eight rats for each time point.After cutting the spinal cord and drawing the blood from inferior vena cava at each time point we detected glutamic acid content in spinal cord and glutamic acid content in serum.At hour 4,8,and 12 following perfusion,evaluated neurological fuction using Tarlov method in model and tanshinone groups.RESULTS All 88 rats were included in the final analysis.Glutamic acid content in spinal and serum of model and tanshinone groups rose ischemia/reperfusion injury 30min later,reached a peak 4 hours after reperfusion,fall-off thenceforth,and put back 12 hours after reperfusion.CONCLUSION Tanshinone could reduce glutamic acid content in spinal cord and protected neurological function in a rat model of spinal cord ischemia/reperfusion injury.

关 键 词：脊髓缺血再灌注损伤 丹参酮 谷氨酸 

分 类 号：R969[医药卫生—药理学]