衣原体噬菌体Chp3衣壳蛋白Vp1二级结构及B细胞抗原表位预测  

Prediction of secondary structure and B-cell epitopes of capsid protein Vp1 from Chlamydiaphage 3

作　　者：姚卫锋[1] 刘原君[1] 李燕[1] 齐蔓莉[1] 田敬群[1] 刘全忠[1] 

机构地区：[1]天津医科大学总医院皮肤科,天津300052

出　　处：《天津医科大学学报》2010年第2期188-190,207,共4页Journal of Tianjin Medical University

基　　金：国家自然科学基金资助项目(30671879)

摘　　要：目的:预测衣原体噬菌体Chp3Vp1蛋白的二级结构和B细胞表位。方法:以Chp3Vp1氨基酸序列为基础,采用Gamier-Robson法、Chou-Fasman法和Karplus-Schulz法预测蛋白二级结构;按Kyte-Doolittle法、Hopp-Woods法、Emini法和Jameson-Wolf法预测蛋白的抗原表位。结果:Chp3Vp1蛋白含多个抗原位点,预测其N端1-10,101-112,159-166,174-184,189-195,288-299,323-333,419-435,477-490为优势表位。结论:Chp3Vp1蛋白有较强的免疫原性,预测的抗原位点为之后蛋白相互作用研究和单抗制备提供了理论依据。Objective： To predict the secondary structure and B cell epitope of capsid protein Vp1 from Chlamydiaphage Chp3. Methods： The secondary structure was predicted by the method of GamierRobson, Chou-Fasman and Karplus-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle,Hopp-Woods,Emini and Jameson-Wolf. Results： The sections of 1-10,101-112, 159- 166,174-184,189-195,288-299,323-333, 419-435 and 477-490 in the N terminal of Chp3Vp1 protein could be the epitope of B cell. Conclusion： Chp3Vp1 protein has strong immumogenicity.The research provides the basis for the preparation of monoclonal antibody and the reference for the discussion for the molecular regulation mechanism of protein to protein.

关 键 词：衣原体噬菌体 Chp3Vp1蛋白 二级结构 细胞表位 

分 类 号：R751.05[医药卫生—皮肤病学与性病学]