银杏叶提取物对模拟高原低氧暴露下大鼠血脑屏障的保护作用  

Protection of ginkgo biloba extract on blood-brain barrier under exposure to hypoxia

作　　者：巩沅鑫[1] 程淑珍 周其全[1] 

机构地区：[1]解放军第三军医大学高原军事医学系高原疾病学教研室,重庆400038 [2]黑龙江省珍宝岛制药有限公司,黑龙江哈尔滨158400

出　　处：《临床军医杂志》2010年第3期327-329,共3页Clinical Journal of Medical Officers

基　　金：第三军医大学高原系本科生创新基金资助;军队十一五攻关课题资助(08G093)

摘　　要：目的观察银杏叶提取物(GBE)对模拟高原低氧暴露下大鼠血脑屏障的保护作用,并初步阐明其机制。方法将48只SD大鼠随机分为4组,低压舱模拟海拔高度7000m连续减压缺氧3d制备大鼠实验性高原脑水肿模型。GBE前期干预组在缺氧前3d按60mg/(kg.d)给予GBE干预处理,GBE后期干预组在缺氧开始24h后按相同剂量给予GBE至减压缺氧结束,以湿干比值法评价其对脑含水量的影响,硝酸镧示踪法观察GBE对低氧暴露下血脑屏障通透性的影响。结果 GBE前期干预组和GBE后期干预组大鼠脑含水量和硝酸镧颗粒渗出均明显少于高原对照组(P<0.01),且GBE前期干预组的效果显著优于GBE后期干预组(P<0.01)。结论 GBE对低氧暴露下血脑屏障通透性增高具有一定保护作用。Objective To observe the protective effect of ginkgo biloba extract（GBE）on blood-brain barrier of plateau model rats with vasogenic brain edema,and to stated its mechanism initially.Methods Forty-eight SD rats,which were made into experimental models with high altitude（7,000 meters）cerebral edema by offering decompressional and hypoxic surroundings in low pressure chamber for 3 days,were randomly divided into 4 groups.GBE pretreated group was given GBE at the standard of 60 mg/kg/d on the first three days of hypoxia,delayed GBE treated group was given GBE at the same dose at the time of 24 hours after hypoxia to the end of decompression hypoxia.We evaluated the influence of GBE on brain water content by exposing to hypoxia with the method of gaining wet ratio,and by observing the influence of GBE on blood-brain barrier permeability by exposing to hypoxia with the anthanum nitrate tracer method.Results GBE pretreated group and delayed GBE treated group were significant decreased in the brain water content and lanthanum nitrate particles,compared with the other groups（P〈0.01）,and the effect of GBE pretreated group was better than delayed GBE treated group（P〈0.01）.Conclusion GBE has some protective effect against the increase in blood-brain barrier permeability due to exposure to hypoxia.

关 键 词：银杏叶提取物 低氧暴露 血脑屏障 保护作用 

分 类 号：R972[医药卫生—药品]