短期持续高糖输注诱导胰岛β细胞增殖的大鼠模型建立  

Establishment of a Rat Model of Pancreatic β-Cells Proliferation with Short-term Concentrated Glucose Solution Infusion

作　　者：张宏利[1] 李文毅[1] 李凤英[1] 龙红梅[1] 刘赟[1] 吴铃[1] 张翠平[1] 李果[1] 罗敏[1] 

机构地区：[1]上海市内分泌代谢病研究所上海交通大学附属瑞金医院内分泌代谢病科上海市内分泌代谢病临床医学中心,200025

出　　处：《放射免疫学杂志》2010年第3期318-321,共4页Journal of Radioimmanology

基　　金：国家自然科学基金(30800537)资助项目

摘　　要：目的:建立高糖输注诱导胰岛β细胞增殖的大鼠模型,为研究胰岛β细胞增殖的机制提供研究平台。方法:SD大鼠颈静脉插管后用输液泵连续输注72h的50%葡萄糖和0.45%的NaCl,用BrdU掺入和标记增殖蛋白ki67检测大鼠胰岛β细胞增殖情况,并检测增殖相关基因的表达。结果:输注50%葡萄糖组的大鼠与NaCl组比较胰岛β细胞BrdU和ki67阳性细胞明显增多,增殖相关基因ccnd2,c-myc表达增高,P27降低。结论:通过持续的高糖输注72h可建立胰岛β细胞增殖的大鼠模型。Objective To establish a rat model of pancreatic β-cells proliferation with short-term concentrated glucose solution infusion.Methods SD rats were infused for 72h with either 50% glucose or 0.45% NaCl via an inwelling jugular catheter.Prolferation of pancreatic β cell was detectd by staining for expression of BrdU and ki67 on pancreas islet cell sections.The mRNA expression of proliferation genes were also analysed.Results Pancreas sections stained for BrdU/ki67 and insulin showed a marked increase in the number of BrdU/ki67-positive β-cells in glucose-infused rat compared with NaCl-infused rat.In islet cells of glucose-infused rat ccnd2 and c-myc were upregulated and P27 downregalated.Conclusion We have successfully established a model to induce rat β-cells proliferation in vivo.

关 键 词：胰岛Β细胞 增殖 葡萄糖 

分 类 号：R64[医药卫生—外科学]