核桃胚营养代谢盛期总RNA提取方法研究  

METHOD RESEARCH OF EXTRACTING TOTAL RNA FROM WALNUT EMBRYO IN NATRATIONALMETABOLIC ACTIVE STAGE

作　　者：孙翠[1] 李永涛[1] 曲文文[1] 陈兴彬[1] 杨克强[2] 

机构地区：[1]山东农业大学农业生态与环境重点实验室,中国泰安271018 [2]作物生物学国家重点实验室,中国泰安271018

出　　处：《山东农业大学学报（自然科学版）》2010年第2期175-180,共6页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：山东省农业良种工程项目(2008LZ010);山东省自然基金(2007ZRB01554);作物生物学国家重点实验室开放项目(2009KF02);山东省教育厅(J07WF06)项目资助

摘　　要：核桃是21世纪的超级食品。对核桃胚总RNA的提取是开展核桃胚发育和代谢分子生物学研究的基础环节。本试验针对核桃营养代谢盛期胚的特点,通过对改进的CTAB法、常规的CTAB法、TRIZOL法和通用植物总RNA提取试剂盒(Bioteke)四种不同总RNA提取方法的比较与分析,表明采用改良CTAB法提取的总RNA完整性较其他方法好,28 S、18 S、5 S条带清晰无明显降解,OD260/OD280值为1.875,无蛋白质污染,易于进行RT-PCR和cDNA的合成,是核桃胚营养代谢盛期的总RNA提取的有效方法。Walnut is called as super food of the 21 of century.Extracting total RNA from walnut embryo is the basic procedure of molecular research on development and metabolism of walnut embryos.The four different methods were used to extract total RNA from walnut embryo： CTAB,TRIZOL Kit,Bioteke Kit and modified CTAB method.The total RNA was extracted by modified CTAB method which was shared 28 S,18 S,and 5 S clear band in agarose gel electrophoresis and the value of OD260 / OD280 was 1.875.The total RNA was extracted by modified CTAB method was easy to carry out RT-PCR test and easy to synthesis cDNA also.The results showed that the improved CTAB method was the effective method to extract total RNA from walnut embryo.

关 键 词：核桃种胚 营养代谢 总RNA提取 改良CTAB法 

分 类 号：S664.1[农业科学—果树学]