“不知火”杂柑叶片黄化机理初探(英文)  

Preliminary Study on the Mechanism of Leaf Chlorosis of"Shiranuhi"

作　　者：肖家欣[1,2] 胡娟[1] 陈世林 周守标[1] 张绍铃[2] 

机构地区：[1]安徽师范大学生命科学学院重要生物资源保护与利用研究安徽省重点实验室,芜湖241000 [2]南京农业大学园艺学院,南京210095 [3]湖北省宜昌市夷陵区农业局,宜昌443100

出　　处：《安徽师范大学学报（自然科学版）》2010年第2期153-158,共6页Journal of Anhui Normal University(Natural Science)

基　　金：Anhui Provincial Natural Science Foundation(070411004);China Postdoctoral Science Foundationfunded project(20080430172);The foundation of Provincial Key Lab of Biotic Environment and Ecological Safetyin Anhui(2004sys003)

摘　　要：应用电感耦合等离子发射光谱仪、H-600型透射电子显微镜研究了"不知火"杂柑叶片异常黄化及其对解剖特征的影响。主要结果表明:"不知火"黄化叶的Zn、Mg和Ca含量均处于低量水平,且均显著低于"柑";而其SOD、POD和CAT活性、MDA含量均相对高于其正常叶."不知火"黄化叶叶肉细胞的细胞核核膜模糊降解,叶绿体数目明显减少,一些叶绿体形态发生肿胀、呈球形,类囊体片层结构模糊,叶绿体基质中质体小球数量显著增多,细胞质中出现了多泡体.本文还就"不知火"叶片异常黄化的原因及其对解剖特征的影响进行了讨论.Leaf abnormal chlorosis of ＂shiranuhi＂（Citrus reticulata ×（C.reticulata × C.sinenesis））and its effects on anatomical features were investigated with inductively coupled plasma optical emission spectrometry and H-600 transmission electron microscope.The main results showed that Zn,Mg and Ca concentrations in chlorotic leaves from＂shiranuhi＂were at low levels and significantlylower than those of＂ponkan＂（C.reticulata）,while superoxide dismutase,peroxidase and catalase activities,malondialdehyde contents were relatively highinchloroticleaves compared with its normal leaves.In mesophyll cells of chlorotic leaves from＂shiranuhi＂,the number of chloroplast significantly decreased,nuclear envelope became blurred,some chloroplasts swelled and their shapes turnedto round,thylakoid lamellar structures were vague,and large numbers of plastoglobuli were foundin stroma of chloroplast,several multivesicular bodies were additional localized in cytoplasm.Causes of leaf abnormal chlorosis of＂shiranuhi＂and its effects on anatomical features were discussed herein.

关 键 词：“不知火”杂柑 叶片黄化 矿质元素含量 生理指标 解剖及超微结构 

分 类 号：Q945[生物学—植物学] S666[农业科学—果树学]