SAMP8鼠背海马synaptotagminⅣ的年龄效应与分布  

Distribution and age-related changes of synaptotagmin Ⅳ in dorsal hippocampus of SAMP8 mice

作　　者：王龙海[1,2] 陈贵海[2] 杨启纲[2] 张琼[2] 

机构地区：[1]安徽中医学院解剖学教研室,合肥230038 [2]安徽医科大学第一附属医院神经内科

出　　处：《山西医科大学学报》2010年第6期497-500,578,共5页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(30872730);安徽省高校省级自然科学研究重大项目(ZD2008007.1)

摘　　要：目的探讨突触前囊泡蛋白synaptotagmin(Syt)Ⅳ在SAMP8鼠背海马的年龄相关性改变及其分布状况。方法选取三个年龄段SAMP8鼠(青年组4.5月龄22只,中年组9月龄23只,老年组13月龄19只)作为研究对象,制作组织芯片。采用SP免疫组化法检测小鼠背海马SytⅣ的表达。显微成像系统拍摄图像并利用图像分析系统Image-ProPlus6.0进行光密度值测定。结果 4.5月龄SAMP8鼠背海马CA3、CA1和齿状回中SytⅣ的相对含量显著高于13月龄(P<0.05),也高于9月龄鼠(P<0.05);9月龄高于13月龄鼠,但差异无统计学意义(P>0.05)。SytⅣ在不同年龄组SAMP8鼠背海马不同亚区中均有表达,且以锥体细胞和颗粒细胞胞膜表达较高。结论 SAMP8鼠背海马SytⅣ水平随年龄增加而降低。SytⅣ可能是小鼠背海马锥体细胞和颗粒细胞的轴-体或树-体式突触中的主要突触囊泡蛋白。Objective To explore the distribution and age-related changes of synaptotagmin（Syt） Ⅳ in dorsal hippocampus of SAMP8 mice. Methods The SAMP8 mice were divided into 3 groups：young group（ aged 4.5 months）,middle-age group（aged 9 months）and old group（aged 13 months）.The expression of Syt Ⅳ was detected by the immunohistochemistry method（SP method）. Results The relative Syt Ⅳ amount in the 4.5-month-old mice was significantly higher than that in the elder mice（P0.05）.Syt Ⅳ expressed in both the pyramidal and the granular cells in hippocampus in different age mice.Meantime,the Syt Ⅳ expression was higher in the membranes of pyramidal and granular cells than that in other subareas of each sample. Conclusion The results indicate that the dorsal hippocampal Syt Ⅳ decreases with aging,and the Syt Ⅳ may be the main synaptotagmin in the axo-and dendro-somatic synapses of pyramidal and granular cells in dorsal hippocampus.

关 键 词：衰老 小鼠 突触结合蛋白Ⅳ 组织芯片 

分 类 号：R338.1[医药卫生—人体生理学]