白纹阴阳竹试管快繁技术研究  

A Study on Tissue Culture Propagation Technology of Hibanobambus tranguillans

作　　者：曾余力[1] 王旭锋[1] 林新春[1] 桂仁意[1] 张翠萍[1] 黄丽春[1] 

机构地区：[1]浙江林学院浙江省现代森林培育重点实验室,浙江临安311300

出　　处：《西南林学院学报》2010年第3期38-41,共4页Journal of Southwest Forestry College

基　　金：浙江省科技厅重大科技专项(2006C12008)资助;浙江省林业厅项目(08A09)资助

摘　　要：以白纹阴阳竹的嫩芽为外植体,对其试管繁殖进行研究。结果表明：取材前7天对材料喷施农药（早上喷施链霉素1.00 g/L,傍晚喷施恶霉灵0.25 g/L）,可降低外植体污染率并提高成活率,污染率和成活率分别为21.17%和87.90%;以MS＋5 mg/L BA＋0.002 mg/L TDZ为基本培养基,接种5芽/丛,增殖效果最佳,增殖系数达3.40,新芽粗壮;在增殖培养基中添加0.5-1.0 g/L香蕉粉,接种3芽/丛,增殖系数达5.67。水解酪蛋白、麦芽提取物、酵母提取物都不利于其生长增殖;采用两阶段诱导白纹阴阳竹生根,以100 mg/L IBA处理7 d最佳,生根率100%。A tissue culture experiment with Hibanobambus tranguillans was conducted by taking the young axillary buds as explants.The results showed that the pretreatment with spraying pesticides（streptomycin 1.00 g/L,hymexazol 0.25 g/L） on the mother plants in the morning 7 days before picking the young buds for tissue culture could effectively reduce contamination incidence and increase the survival rate.The final contamination rate and survival rate were 21.17% and 87.90% respectively.The best proliferation was obtained by planting 5 buds per cluster in MS ＋ 5 mg/L BA ＋0.002 mg/L TDZ basal medium,the proliferation coefficient was up to 3.40 with vigorous growing of the new shoots.When 0.5-1.0 g/L banana powder was supplemented to the proliferation medium and 3 buds per cluster were transplanted,the proliferation coefficient could reach 5.67.It was showed that other addenda including casein hydrolysate,malt extract or yeast extract did not help the growth and proliferation.Two-step rooting method,i.e.,to treat the shoots with 100 mg/L IBA for 7 days,and then transfer them into IBA free MS medium could obtain 100% of rooting rate.

关 键 词：白纹阴阳竹 快繁 丛生芽 有机添加物 

分 类 号：S795.904[农业科学—林木遗传育种]