用聚合酶链反应检测羊流产衣原体的初步研究  

A Preliminary Study on the Detection of Ovine Abortion Chlamydia by Polymerase Chain Reaction

作　　者：苗振川 哈斯阿古拉 赵亚芳[1,2] 张宝发[1,2] 郭志成 张鹤龄[1,2] 

机构地区：[1]内蒙古畜牧科学院 [2]内蒙古大学生物工程中心

出　　处：《中国兽医杂志》1999年第2期3-4,共2页Chinese Journal of Veterinary Medicine

摘　　要：依据鹦鹉热衣原体羊流产株的主要外膜蛋白基因核苷酸顺序，设计、合成１对ＰＣＲ引物。利用该对引物，以衣原体基因组ＤＮＡ为模板，可以用ＰＣＲ扩增出一１．１７ＫｂＤＮＡ片段。检测灵敏度可达０．１ｐｇ。采集衣原体感染的山羊流产胎衣等病料制取核酸样品，进行ＰＣＲ检测，同样扩增出特异性条带。作为对照的健康动物组织、常见病原菌的核酸样品则都呈阴性反应。用建立的ＰＣＲ检测衣原体的方法检查了６５份自然病料。According to the nucleotide sequence of the major outer membrane protein gene of the ovine abortion strain of Chlamydia psittaci, a pair of primer were synthesized and employed in polymerase chain reaction (PCR). A specific 1.1Kb DNA fragment was amplified from Chlamydial genomic DNA templet. As a detection method,its sensibility degree was 0.1pg. The same specific amplified fragment also occurred in PCR detection when nucleic acid samples extracted from Chlamydia infected goat tissues was as templet. By contrast, the controls with nucleic acid samples from healthy animal tissues or frequent pathogenic bacteria were negitive. Using the established PCR detection method, We detected 65 samples collected from natrural field, out of which 6 samples turned out to be positive.

关 键 词：羊流产衣原体 PCR检测 检测方法 

分 类 号：S858.262.6[农业科学—临床兽医学] S854.43[农业科学—兽医学]